Detailed information of TA system TA222331

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1503492..1505091
• Replicon: chromosome
• Accession: NZ_CP085970
• Organism: Bordetella pertussis strain FDAARGOS_1542

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: Q7VVM6
• Locus tag: LK412_RS07200
• Protein ID: WP_003816876.1
• Coordinates: 1503751..1505091 (+)
• Length: 447 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LK412_RS07195
• Protein ID: WP_019247974.1
• Coordinates: 1503492..1503749 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK412_RS07180 (LK412_07180)	1499155..1499766	-	612	WP_010931041.1	DUF2239 family protein	-
LK412_RS07185 (LK412_07185)	1499989..1501449	+	1461	WP_003812368.1	IMP dehydrogenase	-
LK412_RS07190 (LK412_07190)	1501546..1503138	+	1593	WP_010931040.1	glutamine-hydrolyzing GMP synthase	-
LK412_RS07195 (LK412_07195)	1503492..1503749	+	258	WP_019247974.1	helix-turn-helix transcriptional regulator	Antitoxin
LK412_RS07200 (LK412_07200)	1503751..1505091	+	1341	WP_003816876.1	type II toxin-antitoxin system HipA family toxin	Toxin
LK412_RS07205 (LK412_07205)	1505187..1505876	-	690	WP_010931038.1	TetR/AcrR family transcriptional regulator	-
LK412_RS07210 (LK412_07210)	1505889..1507319	+	1431	WP_019247976.1	MFS transporter	-
LK412_RS07215 (LK412_07215)	1507551..1508629	+	1079	Protein_1432	tellurite resistance/C4-dicarboxylate transporter family protein	-
LK412_RS07220 (LK412_07220)	1509062..1509865	+	804	WP_019248072.1	IclR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (10-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-70)

Sequences

Toxin

Protein

• Length: 447 a.a.
• Molecular weight: 49632.79 Da
• Isoelectric Point: 7.4158

>T222331 WP_003816876.1 NZ_CP085970:1503751-1505091 [Bordetella pertussis]
MGRRSHSRSLSIWTNGVRVGRWTVPARGEMELQYDVDWVRADVGRPLSLSLPFNLQNLPLKGERVANYFDNLLPDSDAIR
RRVAERFRTGSVDPFDLLKAIGRDCVGAVQILEDEEIPEGFDRIEGVPLSEEDIERHLIETVTPRAFAAGRDPDADFRIS
LAGAQEKTAFLWWGGQWLAPRGATSTSHIFKLPLGLVGGRRADFSTSVDNEWLCLKLLKAYGLDVAEASIATFGQQRVLV
VERFDRRVAPNGQWLMRLPQEDFCQVEGCSPLRKYENEGGPGLKALFATLRQSVNAAVDMRTLMAAQILFWLLRAPDGHA
KNFSIQLLPRGRFQLTRLYDVMSAYPVLGDGANQWSPFEIKLAMALIGKNRHYEMHGIQRRHFNSTAQKVGYGPTAEPII
EDLLARTPAIIAAVQADLPQDFSPRVADSVLGGLAQAAKSLGAMMP

Nucleotide

• Length: 1341 bp

>T222331 NZ_CP117815:c52316-52119 [Legionella pneumophila]
ATGAAAAGCGCAGAAATAATAAAAATAATTGAACGCGATGGTTGGGTGCAATGTCATCAGAAAGGAAGTCATTGCCAATT
TAAACATCCCACCAAAAAGGGTAGGGTGACAGTACCTCATCCTAAAAAAGATTTACCTATAAAAACAGTGGCCAGTATCT
TTAAACAAGCTGGTATTAATAAGGAGGATATAAAATGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8815.04 Da
• Isoelectric Point: 7.1642

>AT222331 WP_019247974.1 NZ_CP085970:1503492-1503749 [Bordetella pertussis]
MPPPSQPLLTTGQLGAVLQAARKAQGLTQSALASRIGLSQSRVSHLELNAHQLSVEQLLAWCAALGLELTIATRGSPAGS
SDADW

Nucleotide

• Length: 258 bp

>AT222331 NZ_CP117815:c52122-51718 [Legionella pneumophila]
ATGAGATATCCTGTAGTAATACATAAAGACGAACATAGTGATTTTGGTGTAATTGTCCCTGATATTCCTGGATGCTATTC
TTCTGGTAGCACTTATGATGAAGCTTTAACTAACGTAATTGAGGCAATTGAATGCCATTTGGAAGGATTATTAATAGATA
ATGAGCCTCTTCCAGTTGGTACAACAATTGACCGATGGATTAATGATGAAGAGTTTCAGGGCGGGGTATGGGCATTTGTT
GACATTGATTTAAGCCAAATATCGGGTAAAGCAAAACGCATTAATATCACTATGCCAGAGCGCATTTTAAGCCTCATCGA
TCTGTATGCCAAAAACCATGCGATAAAAAATCGTTCCTCCTTTTTGGCCGATGCAGCTCTGAGTTATATGGAAAGTCATA
AGTAA

Structures

Toxin

• AlphaFold DB: Q7VVM6