Detailed information of TA system TA222324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 2177752..2178235
• Replicon: chromosome
• Accession: NZ_CP085960
• Organism: Corynebacterium diphtheriae strain FDAARGOS_1552

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A806GHY4
• Locus tag: LK405_RS10415
• Protein ID: WP_014302726.1
• Coordinates: 2177972..2178235 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: LK405_RS10410
• Protein ID: WP_003850117.1
• Coordinates: 2177752..2177988 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK405_RS10385 (LK405_10385)	2173131..2173529	+	399	WP_003850107.1	LysE family transporter	-
LK405_RS10390 (LK405_10390)	2173795..2174553	+	759	WP_003850109.1	hypothetical protein	-
LK405_RS10395 (LK405_10395)	2174833..2175681	+	849	WP_003850110.1	helix-turn-helix domain-containing protein	-
LK405_RS10400 (LK405_10400)	2175603..2176355	+	753	WP_227889257.1	IS30 family transposase	-
LK405_RS10405 (LK405_10405)	2176566..2177249	-	684	WP_014302723.1	hypothetical protein	-
LK405_RS10410 (LK405_10410)	2177752..2177988	+	237	WP_003850117.1	ribbon-helix-helix domain-containing protein	Antitoxin
LK405_RS10415 (LK405_10415)	2177972..2178235	+	264	WP_014302726.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LK405_RS10420 (LK405_10420)	2178362..2178655	+	294	WP_014302727.1	RNA-binding S4 domain-containing protein	-
LK405_RS10425 (LK405_10425)	2178655..2179479	+	825	WP_014301205.1	VOC family protein	-
LK405_RS10430 (LK405_10430)	2179485..2179790	+	306	WP_003850125.1	MGMT family protein	-
LK405_RS10435 (LK405_10435)	2179800..2180720	-	921	WP_003850127.1	alpha/beta hydrolase	-
LK405_RS10440 (LK405_10440)	2180860..2181486	+	627	WP_014318472.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2143766..2178655	34889

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (7-43)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9911.45 Da
• Isoelectric Point: 10.9201

>T222324 WP_014302726.1 NZ_CP085960:2177972-2178235 [Corynebacterium diphtheriae]
VAWEISFSPRAVKSFKKLDTGEQRRVSKFLREVGALEDPRLRGKALTANKSGLWRWRVGDYRIIADIVDARVVVVVVDVG
HRSKAYD

Nucleotide

• Length: 264 bp

>T222324 NZ_CP117811:2002578-2002847 [Lentisphaera profundi]
ATGCTTGAACTCGTATATGGTAGTCAATTCAAAAAGGATTTTAAGAAAGCTCGTAAGCTTCCGATTGAAGATTTAAGCAA
CATTTTCAAAATCATATCGACACTTCAAAGTCAAACGCCTTTAGACCAAAAGTACAAAGACCATGATCTAACTGGAAACT
ATAAAGCTTATCGGGAATGTCATATTAAACCCGACCTACTTTTAATTTATACAGTCGATGGTAAAGATTTATCATTGGTT
AGAATCGGGTCTCATTCAGAATTATTTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8911.02 Da
• Isoelectric Point: 4.8604

>AT222324 WP_003850117.1 NZ_CP085960:2177752-2177988 [Corynebacterium diphtheriae]
MSGSVISLRIDDEQKRRLDELSRRTGRPCSFYLREALSAHLADLEYVYRLEEEAAQVRRGELGTVPLSQLERECGLGD

Nucleotide

• Length: 237 bp

>AT222324 NZ_CP117811:2002310-2002585 [Lentisphaera profundi]
ATGAAAACGGAATTGATTAGTACTAGAATCGAACATGACTTAAAAGTTGAGTTCACCAACATCTGTGATGATTTAGGTTT
AAGTCCATCACAAGCTATTAAACTGTTTGCTAAAGCTGTTATCAACTATGGCGGTATTCCCTTCCAGTTAAAAGTTAAGC
AACCTAACGCTGTAACTCGTCAAGCTATGGAAGAGTTAGAAAGTGGCAAGGGAACTAGAGTCAAGGATGCCACAGAACTA
TTCAGTGACTTGGGTGTAGATATTCAGGATGCTTGA

Structures

Toxin

• AlphaFold DB: A0A806GHY4