Detailed information of TA system TA222322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 1859420..1859903
• Replicon: chromosome
• Accession: NZ_CP085959
• Organism: Corynebacterium diphtheriae strain FDAARGOS_1553

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6J4WN23
• Locus tag: LK425_RS08635
• Protein ID: WP_014307502.1
• Coordinates: 1859634..1859903 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A6J4WZ27
• Locus tag: LK425_RS08630
• Protein ID: WP_014307503.1
• Coordinates: 1859420..1859653 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK425_RS08605 (LK425_08605)	1854504..1855301	+	798	WP_014304056.1	ABC transporter ATP-binding protein	-
LK425_RS08610 (LK425_08610)	1855558..1855859	+	302	Protein_1679	FtsX-like permease family protein	-
LK425_RS08615 (LK425_08615)	1856051..1856629	+	579	WP_014311015.1	restriction endonuclease subunit S	-
LK425_RS08620 (LK425_08620)	1856724..1858055	+	1332	WP_014307506.1	ATP-binding protein	-
LK425_RS08625 (LK425_08625)	1858083..1858682	+	600	WP_041627054.1	RloB family protein	-
LK425_RS08630 (LK425_08630)	1859420..1859653	+	234	WP_014307503.1	ribbon-helix-helix protein, CopG family	Antitoxin
LK425_RS08635 (LK425_08635)	1859634..1859903	+	270	WP_014307502.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LK425_RS08640 (LK425_08640)	1860292..1862103	-	1812	WP_014311013.1	N-acetylmuramoyl-L-alanine amidase	-
LK425_RS08645 (LK425_08645)	1862237..1862389	+	153	Protein_1686	iron chelate uptake ABC transporter family permease subunit	-
LK425_RS08650 (LK425_08650)	1862527..1863621	-	1095	WP_014311011.1	HigA family addiction module antitoxin	-
LK425_RS08655 (LK425_08655)	1863636..1863935	-	300	WP_041627052.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LK425_RS08660 (LK425_08660)	1864365..1864505	+	141	WP_014311009.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1835519..1863935	28416

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10338.92 Da
• Isoelectric Point: 10.6099

>T222322 WP_014307502.1 NZ_CP085959:1859634-1859903 [Corynebacterium diphtheriae]
VTSWTIEFSPRAAKELRKLDRPVQKRIVAYLREISTLPNPQMRGKALTGNWAGFWRWRVGDYRLVAAIEDDRVVIVVVSI
GHRSQVYED

Nucleotide

• Length: 270 bp

>T222322 NZ_CP117811:31384-31650 [Lentisphaera profundi]
ATGATTAAAAAATTCTCTGACGATGCCTGGGACGATTATGTTTATTGGCAGACTCAAGATAAAAAGACACTCAAGCGAAT
AAACCTTTTGATCAAAGATACTGAGCGAGATCCATTTTCAGGGATCGGTAAACCAGAGCCACTTAAACATAATCTGAGTG
GTTCTTGGTCACGTAGAATAGATGAAAAAAACCGCATGGTTTACCGGATTAAAAAAGAAGGCGATCAAGAAATTTTAGAG
ATAGCGGCTTTGCGTTATCACTATTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9002.99 Da
• Isoelectric Point: 4.3904

>AT222322 WP_014307503.1 NZ_CP085959:1859420-1859653 [Corynebacterium diphtheriae]
MSNPVISLRIGASEKERLDELSRRTGRSGSFYIREALEKHLDELEYVYGVRSEYEAIRRGELETINLDEVERDLLDD

Nucleotide

• Length: 234 bp

>AT222322 NZ_CP117811:31134-31391 [Lentisphaera profundi]
ATGCAGACGATTACCTACACAAAAGCAAGAAACAACCTCGCTAAAGAAATGGAAAGAGTGAGTTCAGATCACGATTATAC
GGTGATAACCCGAGCTAATCAGGAGAGTGCAGTGCTGATGTCTTTGGAAGATTTTTCATCTTGGCAAGAAACGGCGTATT
TACTTTCGAGTCCAAGTAATGCAAAACGCTTGATGCGTTCACTTGAACAACTCGATAGTGGCGGAGGCCAAGTTAGGGAA
CTTATAGAGGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A6J4WN23

Antitoxin

• AlphaFold DB: A0A6J4WZ27