Detailed information of TA system TA222320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-pasA/RelE-HTH
• Location: 1693592..1694075
• Replicon: chromosome
• Accession: NZ_CP085958
• Organism: Corynebacterium diphtheriae subsp. diphtheriae strain FDAARGOS_1554

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A806GHY4
• Locus tag: LK418_RS08215
• Protein ID: WP_014302726.1
• Coordinates: 1693592..1693855 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: LK418_RS08220
• Protein ID: WP_003850117.1
• Coordinates: 1693839..1694075 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK418_RS08190 (LK418_08190)	1690341..1690967	-	627	WP_014318472.1	hypothetical protein	-
LK418_RS08195 (LK418_08195)	1691107..1692027	+	921	WP_003850127.1	alpha/beta hydrolase	-
LK418_RS08200 (LK418_08200)	1692037..1692342	-	306	WP_003850125.1	MGMT family protein	-
LK418_RS08205 (LK418_08205)	1692348..1693172	-	825	WP_014301205.1	VOC family protein	-
LK418_RS08210 (LK418_08210)	1693172..1693465	-	294	WP_014302727.1	RNA-binding S4 domain-containing protein	-
LK418_RS08215 (LK418_08215)	1693592..1693855	-	264	WP_014302726.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LK418_RS08220 (LK418_08220)	1693839..1694075	-	237	WP_003850117.1	ribbon-helix-helix domain-containing protein	Antitoxin
LK418_RS08225 (LK418_08225)	1694578..1695261	+	684	WP_014302723.1	hypothetical protein	-
LK418_RS08230 (LK418_08230)	1695472..1696224	-	753	WP_227889257.1	IS30 family transposase	-
LK418_RS08235 (LK418_08235)	1696146..1696994	-	849	WP_003850110.1	helix-turn-helix domain-containing protein	-
LK418_RS08240 (LK418_08240)	1697274..1698032	-	759	WP_003850109.1	hypothetical protein	-
LK418_RS08245 (LK418_08245)	1698298..1698696	-	399	WP_003850107.1	LysE family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (7-43)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9911.45 Da
• Isoelectric Point: 10.9201

>T222320 WP_014302726.1 NZ_CP085958:c1693855-1693592 [Corynebacterium diphtheriae subsp. diphtheriae]
VAWEISFSPRAVKSFKKLDTGEQRRVSKFLREVGALEDPRLRGKALTANKSGLWRWRVGDYRIIADIVDARVVVVVVDVG
HRSKAYD

Nucleotide

• Length: 264 bp

>T222320 NZ_CP117798:c4187536-4186223 [Proteus mirabilis]
ATGGCGAGCCTTGATGTGTATATGAATGAATATTATGTGGGTATTTTTACTAAAAAAAGCTCAGGTGCGCATTCATTCCA
ATATGCTGAAGATTGGGTTAATCAACCAGGAAGTCGCCCTATATCTCTTTCTATGCCACTACAACTTCAAGAATATCAAG
GAGATTGTGTCTATAATTTCTTTGATAATCTTTTACCTGATAATCCTGAAATTAGAAATCGTATTGTGAGTCGCTATAAT
GCAAACTCAAATCAACCATTTGATCTATTAACTGCCGTGGGTGCAGATACGGTTGGTGCATTACGGTTACTTCCTAGTAG
TAGTACGCCAGCTAATATAAAAAAAATAGAATATAACAAACTTGATAACGCTGAACTTGAAAAAATACTATTAGGATATC
AATCCAATACACCACTCGGTATGCTTGAAGAATATACTGATTTCAGAATTTCTATCGCTGGAGCGCAAGAAAAAACAGCA
TTACTTTTAAAAGATGATCATTGGTGTGTGCCACACAATAATACGCCGACTACACATATTATTAAGCTACCCATTGGTGT
TATTGAAGGGCATTCCTATACAATGGATTTATCCGATAGTGTAGAAAATGAATATCTTTGTACCTTAATTGCCAAAGAAT
TTGATCTTCCTGTTCCTCACTGTTTTATTGTTAAAACAGAAAATATTAAAGCGCTTGCTGTAGAACGCTTTGATAGAAAA
TACTCATCAGATAAATCGTGGATAATCCGCTTACCCCAAGAAGATTTTTGCCAAATTCTAAATATACCTTCAGCTCTAAA
ATATGAAAATGAAGGGGGGCCTGGCATTATTGATATCATGAATTATTTGCTTGGTTCATCAAATGCTGATCAAGACCGTT
TTCATTTTATGAAGGCACAAGTACTATTTTGGCTTTTAGCTGCAACAGATGGGCATGCAAAGAATTTCTCTGTTTTTATC
AATCAAGAAGGACGTTTTCACCTCACGCCTCTTTATGACATCATGTCTATCTATCCCAATTTTGGAGGACGAGGACTTAA
TCCACGAGATGCAAAACTCGCTATGGGATTACAGGCAAGCCGAGGCAAAAAATATGCCATAGAGCAAATTTTTCCTCGCC
ATTTTTACCAAACAGCTGAAGCAGCCAGTTTCGATCCTTCATTGATGACTGATATTCTTCGTTATTTTTATAAAGAAATG
GACGATGCCATCAAGCGCGTAAAACAACAACTACCTGATGATTTTCCTTCTGAAATTAGCAGTAAAATATTGAATGGAAT
GAAAACACGCTCCGCAAGATTAGCCAATATTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8911.02 Da
• Isoelectric Point: 4.8604

>AT222320 WP_003850117.1 NZ_CP085958:c1694075-1693839 [Corynebacterium diphtheriae subsp. diphtheriae]
MSGSVISLRIDDEQKRRLDELSRRTGRPCSFYLREALSAHLADLEYVYRLEEEAAQVRRGELGTVPLSQLERECGLGD

Nucleotide

• Length: 237 bp

>AT222320 NZ_CP117798:c4187794-4187537 [Proteus mirabilis]
ATGATTATAATTAACGCCTATCAACTCAGCGTCCATCTAAAAGATGTCCGATTAACAAAAAAACTTTCTCAAGGTAAAGT
CGCCCAAAAAGTGGGAATTCGACAAGACACCGTGTCCAATTTTGAACTTAATCCTAATTCCACCAAGCTAGAAACCTTTT
TTAAACTGCTCTCCGCACTAAATTTAGAGATGGAAATTCATCCTAAAGATGCTAAAAATAATTTAGCACACAAAAGTGAA
TGGAAAGAGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A806GHY4