Detailed information of TA system TA222316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1242287..1242936
• Replicon: chromosome
• Accession: NZ_CP085955
• Organism: Finegoldia magna strain FDAARGOS_1557

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LK415_RS06075
• Protein ID: WP_227930496.1
• Coordinates: 1242287..1242466 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B0S3F4
• Locus tag: LK415_RS06080
• Protein ID: WP_012291121.1
• Coordinates: 1242499..1242936 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK415_RS06040 (LK415_06040)	1237475..1239085	+	1611	WP_012291125.1	hypothetical protein	-
LK415_RS06045 (LK415_06045)	1239120..1239797	-	678	WP_012291124.1	hypothetical protein	-
LK415_RS06050 (LK415_06050)	1239821..1240021	-	201	WP_002838001.1	hypothetical protein	-
LK415_RS06055 (LK415_06055)	1240141..1240827	+	687	WP_012291123.1	selenium cofactor biosynthesis protein YqeC	-
LK415_RS06060 (LK415_06060)	1240837..1241130	+	294	WP_002838986.1	cupin domain-containing protein	-
LK415_RS06065 (LK415_06065)	1241319..1241750	+	432	WP_002838046.1	50S ribosomal protein L13	-
LK415_RS06070 (LK415_06070)	1241762..1242154	+	393	WP_002838975.1	30S ribosomal protein S9	-
LK415_RS06075 (LK415_06075)	1242287..1242466	+	180	WP_227930496.1	type II toxin-antitoxin system HicA family toxin	Toxin
LK415_RS06080 (LK415_06080)	1242499..1242936	+	438	WP_012291121.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LK415_RS06085 (LK415_06085)	1243055..1243534	+	480	WP_041250629.1	23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH	-
LK415_RS06090 (LK415_06090)	1243542..1244777	+	1236	WP_012291119.1	CapA family protein	-
LK415_RS06095 (LK415_06095)	1244780..1245862	+	1083	WP_012291118.1	tryptophan--tRNA ligase	-
LK415_RS06100 (LK415_06100)	1246058..1247383	+	1326	WP_012291117.1	glycine/sarcosine/betaine reductase component B subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6610.04 Da
• Isoelectric Point: 11.3008

>T222316 WP_227930496.1 NZ_CP085955:1242287-1242466 [Finegoldia magna]
MTQKEMVKLLKQHGFVEVKSEGKGSHIKMKKPGVRSIPVPKGELKKGTKRQILKQTGLL

Nucleotide

• Length: 180 bp

>T222316 NZ_CP117797:c2482200-2481928 [Fusobacterium nucleatum]
ATGTATGAAATTAAGACCACTACTAAATTTGAAAAAGATTTAAAATTAATGAAAAAAAGAGGATATGATTTAAAATTATT
AAAAGAGGTTATTGATATATTATCTAATGGAGAAGAGTTATCTTCTAAATATAAGGATCATTATTTACAAGGAGATTATA
TTGGTTTTAAAGAATGTCATATAAAGCCTGATTGGTTATTGGTATATAAAATTGATAATAATATACTTGTATTAACACTT
TCAAGAACAGGGAAACATTCAGATTTATTTTAG

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16378.50 Da
• Isoelectric Point: 3.9573

>AT222316 WP_012291121.1 NZ_CP085955:1242499-1242936 [Finegoldia magna]
MLVSYPALFYKEPEGGYFINFPDFEYSGTQGEDISDALFMASDWLGITVSGYIEEGIDLPKVSNINDLSLDGNNPFKDDP
EINLEYVENESFISMICVDVEKYFENTKLVKKTLSIPKWANDLGNRLNLNFSKVLTVAIENLAIK

Nucleotide

• Length: 438 bp

>AT222316 NZ_CP117797:c2482465-2482202 [Fusobacterium nucleatum]
ATGGCTGTTATAAATATTCGTGTTAATGATGAAGTAAAAAAAGAGGCTGAAAGTATCTTTAAATCTTTGGGACTTAATAT
GTCAGTTGCAATGAATTTATTTCTAAAAAAATGTATAAATGAAAATGGAATACCATTTGATTTAAAAATTCCAAATAGAG
AAACAATAGAAGCATTGGAAGAAACTAATAAAATATTAAATGGAAATATAAAAAGAAAATCATATAAAAATGCTGATGAG
CTATTTGAAGATTTAGGTGTGTAA

Structures

Antitoxin

• AlphaFold DB: B0S3F4