Detailed information of TA system TA222315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3045120..3045805
• Replicon: chromosome
• Accession: NZ_CP085954
• Organism: Tsukamurella paurometabola strain FDAARGOS_1558

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LK411_RS15235
• Protein ID: WP_164711568.1
• Coordinates: 3045120..3045518 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LK411_RS15240
• Protein ID: WP_126195936.1
• Coordinates: 3045524..3045805 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK411_RS15200 (LK411_15200)	3041024..3041848	-	825	WP_126195929.1	Rv3717 family N-acetylmuramoyl-L-alanine amidase	-
LK411_RS15205 (LK411_15205)	3041960..3042250	+	291	WP_227967301.1	YbaB/EbfC family nucleoid-associated protein	-
LK411_RS15210 (LK411_15210)	3042261..3042914	+	654	WP_126195931.1	recombination mediator RecR	-
LK411_RS15215 (LK411_15215)	3042918..3043466	+	549	WP_126195932.1	hypothetical protein	-
LK411_RS15220 (LK411_15220)	3043499..3044095	+	597	WP_227966685.1	DinB family protein	-
LK411_RS15225 (LK411_15225)	3044105..3044593	+	489	WP_126195933.1	SRPBCC family protein	-
LK411_RS15230 (LK411_15230)	3044595..3045119	+	525	WP_126195934.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
LK411_RS15235 (LK411_15235)	3045120..3045518	-	399	WP_164711568.1	type II toxin-antitoxin system VapC family toxin	Toxin
LK411_RS15240 (LK411_15240)	3045524..3045805	-	282	WP_126195936.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LK411_RS15245 (LK411_15245)	3046167..3046874	-	708	WP_126198768.1	glutamine amidotransferase	-
LK411_RS15250 (LK411_15250)	3046901..3048148	-	1248	WP_126195937.1	Mur ligase family protein	-
LK411_RS15255 (LK411_15255)	3048187..3048933	+	747	WP_126195938.1	class I SAM-dependent methyltransferase	-
LK411_RS15260 (LK411_15260)	3048820..3049815	-	996	WP_126195939.1	EamA family transporter	-
LK411_RS15265 (LK411_15265)	3049888..3050778	+	891	WP_126195940.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-46)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14196.89 Da
• Isoelectric Point: 4.3553

>T222315 WP_164711568.1 NZ_CP085954:c3045518-3045120 [Tsukamurella paurometabola]
MDTSALTKLIIAEQESDALTVWVAEQAEAHETLTTSDVGRIELMRTAARRDDDEIFDHARYVANWINGASVTEEIVAGAE
HIGPPSLRSLDAIHLASAVSIRELVSAFVSYDKRLLDAARAEGLPVVSPGAA

Nucleotide

• Length: 399 bp

>T222315 NZ_CP117797:312082-312405 [Fusobacterium nucleatum]
GTGGTAGAAAGAGGAGATATTATTATAATAAATTTTGATCCTGTAAAAGGACATGAACAAGCAGGGAAAAGGCCAGCATT
GGTTATCAGTAATGAGAAATTTTATAGAATATTTAAATTAGCAGTAATTCTTCCAATAACAAATAATACAAAAGATTTTC
CATTTCACGTGCTATTAGATGATAGAACAAATATAAAAGGTGCAATATTATGTGAACATTTAAGAACAGTTGATTTAGAA
GAAAGAAAATACAATAAAGTTGAAAAATTACCAGAAAATCTGTTAGAAGAAGTTCTTGAAAAAGTGAGCTTAATTTTTCA
ATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10737.54 Da
• Isoelectric Point: 10.1401

>AT222315 WP_126195936.1 NZ_CP085954:c3045805-3045524 [Tsukamurella paurometabola]
MEQIGVRELRQHASKYLARVEAGEEIEVTNHGRLVARLVPVNEKERQFRELVAAGKIKPAKHPFDLEKLVAMRRPLKPGE
KSLTQILLEMREE

Nucleotide

• Length: 282 bp

>AT222315 NZ_CP117797:311834-312088 [Fusobacterium nucleatum]
ATGGAAGTATTAAGAGTTCAAAAATGGGGAAATAGTCAAGGAATTAGATTACCTAAAAAAATTTTAAAAGATTTAAAAAT
AGATATAAATGATAAAATTGAAATAAGTTTAAATGGAGAAGAAATTATACTAAAAAAAGTAAAAAAATATACTAGTTTAG
ATGATTTATTTAAAGATTATAGAGGAGACTATAGAAAAGATTTTGATGAATTTGAATTTTTTGGAGAAGCACAAGGTCGT
GAATTTAAGTGGTAG