Detailed information of TA system TA222305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 340321..340958
• Replicon: chromosome
• Accession: NZ_CP085952
• Organism: Haemophilus influenzae strain FDAARGOS_1560

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: E4QWH2
• Locus tag: LK401_RS01720
• Protein ID: WP_005649049.1
• Coordinates: 340554..340958 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: E4QWH3
• Locus tag: LK401_RS01715
• Protein ID: WP_005649046.1
• Coordinates: 340321..340557 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK401_RS01690 (LK401_01690)	335700..336440	-	741	WP_005649034.1	YebC/PmpR family DNA-binding transcriptional regulator	-
LK401_RS01695 (LK401_01695)	336452..336925	-	474	WP_011271985.1	dihydroneopterin triphosphate diphosphatase	-
LK401_RS01700 (LK401_01700)	336947..338713	-	1767	WP_011271986.1	aspartate--tRNA ligase	-
LK401_RS01705 (LK401_01705)	338932..339450	+	519	WP_005649040.1	isoprenylcysteine carboxyl methyltransferase family protein	-
LK401_RS01710 (LK401_01710)	339503..340228	+	726	WP_011271987.1	carboxy-S-adenosyl-L-methionine synthase CmoA	-
LK401_RS01715 (LK401_01715)	340321..340557	+	237	WP_005649046.1	antitoxin	Antitoxin
LK401_RS01720 (LK401_01720)	340554..340958	+	405	WP_005649049.1	type II toxin-antitoxin system VapC family toxin	Toxin
LK401_RS01725 (LK401_01725)	341025..341432	+	408	WP_005688980.1	lactoylglutathione lyase	-
LK401_RS01730 (LK401_01730)	341506..342195	+	690	WP_011271988.1	ribonuclease T	-
LK401_RS01735 (LK401_01735)	342509..343861	+	1353	WP_005694344.1	Na+/H+ antiporter family protein	-
LK401_RS01740 (LK401_01740)	343894..344481	+	588	WP_005654063.1	primosomal replication protein	-
LK401_RS01750 (LK401_01750)	344832..345398	-	567	WP_005544066.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-124)

Antitoxin

• SpoVT-AbrB domain (6-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15692.29 Da
• Isoelectric Point: 8.9777

>T222305 WP_005649049.1 NZ_CP085952:340554-340958 [Haemophilus influenzae]
MIYMLDTNIIIYLMKNRPKIIAERVSQLLPNDRLVMSFITYAELIKGAFGSQNYEQSIRAIELLTERVNVLYPNEQICLH
YGKWANTLKKQGRPIGNNDLWIACHALSLNAVLITHNVKEFQRITDLQWQDWTK

Nucleotide

• Length: 405 bp

>T222305 NZ_CP117765:22402-22761 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9037.19 Da
• Isoelectric Point: 4.2342

>AT222305 WP_005649046.1 NZ_CP085952:340321-340557 [Haemophilus influenzae]
MLTKVFQSGNSQAVRIPMDFRFDVDTVEIFRKENGDVVLRPVSKKTDDFLALFEGFDETFIQALEARDDLPPQERENL

Nucleotide

• Length: 237 bp

>AT222305 NZ_CP117765:22761-23060 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA

Structures

Toxin

• AlphaFold DB: E4QWH2

Antitoxin

• AlphaFold DB: E4QWH3