Detailed information of TA system TA222301

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 482938..483586
• Replicon: chromosome
• Accession: NZ_CP085951
• Organism: Haemophilus influenzae strain FDAARGOS_1561

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A0Y7JUP0
• Locus tag: LK431_RS02475
• Protein ID: WP_005694481.1
• Coordinates: 483227..483586 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: LK431_RS02470
• Protein ID: WP_005650837.1
• Coordinates: 482938..483234 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK431_RS02445 (LK431_02445)	478349..478906	-	558	WP_111695309.1	DNA-3-methyladenine glycosylase I	-
LK431_RS02450 (LK431_02450)	478903..479721	-	819	WP_111695308.1	shikimate dehydrogenase	-
LK431_RS02455 (LK431_02455)	479725..480276	-	552	WP_042593999.1	Sua5/YciO/YrdC/YwlC family protein	-
LK431_RS02460 (LK431_02460)	480292..480828	-	537	WP_111695307.1	type I DNA topoisomerase	-
LK431_RS02465 (LK431_02465)	480838..482754	-	1917	WP_111695306.1	ABC transporter ATP-binding protein	-
LK431_RS02470 (LK431_02470)	482938..483234	-	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
LK431_RS02475 (LK431_02475)	483227..483586	-	360	WP_005694481.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LK431_RS02480 (LK431_02480)	483856..486168	-	2313	WP_227894326.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
LK431_RS02485 (LK431_02485)	486376..486774	-	399	Protein_456	TonB-dependent receptor plug domain-containing protein	-
LK431_RS08955	486768..486839	-	72	Protein_457	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 14321.67 Da
• Isoelectric Point: 10.1408

>T222301 WP_005694481.1 NZ_CP085951:c483586-483227 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVKLLSELGTSVGKPYVKHLDGEIWELRPIRDRILFARLM
DGRFVLLHQFMKKTQKTPKREIQTAQQRLSELKERLKNE

Nucleotide

• Length: 360 bp

>T222301 NZ_CP117760:2762-3049 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT222301 WP_005650837.1 NZ_CP085951:c483234-482938 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT222301 NZ_CP117760:3036-3350 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A0Y7JUP0