Detailed information of TA system TA222298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 1445235..1445883
• Replicon: chromosome
• Accession: NZ_CP085949
• Organism: Haemophilus influenzae strain FDAARGOS_1562

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A0Y7JUP0
• Locus tag: LK420_RS06945
• Protein ID: WP_005694481.1
• Coordinates: 1445235..1445594 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: LK420_RS06950
• Protein ID: WP_005650837.1
• Coordinates: 1445587..1445883 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK420_RS06920 (LK420_06920)	1441243..1441407	-	165	WP_021034578.1	hypothetical protein	-
LK420_RS06925 (LK420_06925)	1441460..1441624	-	165	WP_021034577.1	hypothetical protein	-
LK420_RS06940 (LK420_06940)	1441987..1444974	+	2988	WP_061725097.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
LK420_RS06945 (LK420_06945)	1445235..1445594	+	360	WP_005694481.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LK420_RS06950 (LK420_06950)	1445587..1445883	+	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
LK420_RS06955 (LK420_06955)	1446040..1447956	+	1917	WP_021034575.1	ABC transporter ATP-binding protein	-
LK420_RS06960 (LK420_06960)	1447966..1448502	+	537	WP_021034574.1	type I DNA topoisomerase	-
LK420_RS06965 (LK420_06965)	1448518..1449069	+	552	WP_021034573.1	Sua5/YciO/YrdC/YwlC family protein	-
LK420_RS06970 (LK420_06970)	1449073..1449879	+	807	WP_021034572.1	shikimate dehydrogenase	-
LK420_RS06975 (LK420_06975)	1450093..1450656	+	564	WP_021034570.1	DNA-3-methyladenine glycosylase I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 14321.67 Da
• Isoelectric Point: 10.1408

>T222298 WP_005694481.1 NZ_CP085949:1445235-1445594 [Haemophilus influenzae]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVKLLSELGTSVGKPYVKHLDGEIWELRPIRDRILFARLM
DGRFVLLHQFMKKTQKTPKREIQTAQQRLSELKERLKNE

Nucleotide

• Length: 360 bp

>T222298 NZ_CP117751:c236501-236214 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT222298 WP_005650837.1 NZ_CP085949:1445587-1445883 [Haemophilus influenzae]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT222298 NZ_CP117751:c236740-236501 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A0Y7JUP0