Detailed information of TA system TA222295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ(toxin)
• Location: 1516016..1516640
• Replicon: chromosome
• Accession: NZ_CP085947
• Organism: Helicobacter pylori strain FDAARGOS_1563

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LK447_RS07265
• Protein ID: WP_000912776.1
• Coordinates: 1516016..1516282 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B5Z7Q4
• Locus tag: LK447_RS07270
• Protein ID: WP_001134069.1
• Coordinates: 1516269..1516640 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK447_RS07235 (LK447_07235)	1512005..1512772	-	768	WP_000242349.1	ABC transporter ATP-binding protein	-
LK447_RS07240 (LK447_07240)	1512772..1513752	-	981	WP_000921461.1	iron ABC transporter permease	-
LK447_RS07245 (LK447_07245)	1513745..1514599	-	855	WP_000538084.1	SDR family oxidoreductase	-
LK447_RS07250 (LK447_07250)	1514739..1515263	+	525	WP_001135628.1	acyl-CoA thioesterase	-
LK447_RS07255 (LK447_07255)	1515369..1515640	-	272	Protein_1419	type II toxin-antitoxin system YafQ family toxin	-
LK447_RS07260 (LK447_07260)	1515627..1515938	-	312	WP_001134052.1	type II toxin-antitoxin system antitoxin	-
LK447_RS07265 (LK447_07265)	1516016..1516282	-	267	WP_000912776.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
LK447_RS07270 (LK447_07270)	1516269..1516640	-	372	WP_001134069.1	hypothetical protein	Antitoxin
LK447_RS07275 (LK447_07275)	1517043..1519265	-	2223	WP_000716234.1	outer membrane beta-barrel protein	-
LK447_RS07290 (LK447_07290)	1519644..1520278	+	635	Protein_1424	hypothetical protein	-
LK447_RS07295 (LK447_07295)	1520229..1521341	-	1113	WP_000109761.1	hydrogenase formation protein HypD	-
LK447_RS07300 (LK447_07300)	1521347..1521580	-	234	WP_000335445.1	HypC/HybG/HupF family hydrogenase formation chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-88)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10477.52 Da
• Isoelectric Point: 10.1823

>T222295 WP_000912776.1 NZ_CP085947:c1516282-1516016 [Helicobacter pylori]
MLKIKHHILYKKDFKKLVKNGFDDSVLNKVILTLRKKEPLDPQFQDHALKGKWKPFRECHIKPDILLVYLVKDDELILLR
LGSHSELF

Nucleotide

• Length: 267 bp

>T222295 NZ_CP117750:c4410240-4409953 [Enterobacter hormaechei]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGCTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTAAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 14602.53 Da
• Isoelectric Point: 7.2781

>AT222295 WP_001134069.1 NZ_CP085947:c1516640-1516269 [Helicobacter pylori]
MPNTTAKKDYTKYSEKQLFNLIHQLERKIKKMQNDRVSFKEKMAKELEKRDQNFKDKIDALNELLQKISQAFDDKRDCYL
GHKIPNLETQQAMRDALNGENLETIEDFHAWANEVKKEVNAEN

Nucleotide

• Length: 372 bp

>AT222295 NZ_CP117750:c4410549-4410247 [Enterobacter hormaechei]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
CTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCTGTGGGCGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGGATCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Structures

Antitoxin

• AlphaFold DB: B5Z7Q4