Detailed information of TA system TA222290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 4852825..4853478
• Replicon: chromosome
• Accession: NZ_CP085945
• Organism: Mycobacterium intracellulare strain FDAARGOS_1564

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: LK403_RS22450
• Protein ID: WP_014379252.1
• Coordinates: 4852825..4853286 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: LK403_RS22455
• Protein ID: WP_009957321.1
• Coordinates: 4853290..4853478 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK403_RS22425 (LK403_22425)	4848222..4849442	+	1221	WP_014379256.1	acetyl-CoA acetyltransferase	-
LK403_RS22430 (LK403_22430)	4849444..4850970	+	1527	WP_014379255.1	carotenoid oxygenase family protein	-
LK403_RS22435 (LK403_22435)	4850970..4851458	+	489	WP_036458243.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
LK403_RS22440 (LK403_22440)	4851463..4851897	-	435	WP_008253914.1	hypothetical protein	-
LK403_RS22445 (LK403_22445)	4851971..4852741	-	771	WP_014379253.1	VOC family protein	-
LK403_RS22450 (LK403_22450)	4852825..4853286	-	462	WP_014379252.1	SRPBCC family protein	Toxin
LK403_RS22455 (LK403_22455)	4853290..4853478	-	189	WP_009957321.1	antitoxin	Antitoxin
LK403_RS22460 (LK403_22460)	4853544..4855931	-	2388	WP_014379251.1	cation-translocating P-type ATPase	-
LK403_RS22465 (LK403_22465)	4855928..4857505	-	1578	WP_014379250.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16609.18 Da
• Isoelectric Point: 8.5617

>T222290 WP_014379252.1 NZ_CP085945:c4853286-4852825 [Mycobacterium intracellulare]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVAVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T222290 NZ_CP117750:c4002507-4002136 [Enterobacter hormaechei]
ATGGAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTAATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTTATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATTGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCGCAGAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGATTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT222290 WP_009957321.1 NZ_CP085945:c4853478-4853290 [Mycobacterium intracellulare]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT222290 NZ_CP117750:c4002752-4002510 [Enterobacter hormaechei]
ATGAGAGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAATCT
CAAGCAATTGCGAAATCAGCCACGTGCTGAACTGCTTCGGGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: X8A187