Detailed information of TA system TA222289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 4604188..4604752
• Replicon: chromosome
• Accession: NZ_CP085945
• Organism: Mycobacterium intracellulare strain FDAARGOS_1564

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LK403_RS21315
• Protein ID: WP_036458315.1
• Coordinates: 4604188..4604559 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LK403_RS21320
• Protein ID: WP_036458377.1
• Coordinates: 4604546..4604752 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK403_RS21275 (LK403_21275)	4599642..4599851	-	210	WP_080687320.1	hypothetical protein	-
LK403_RS21280 (LK403_21280)	4599998..4600681	+	684	WP_036458379.1	hypothetical protein	-
LK403_RS21285 (LK403_21285)	4600729..4601052	-	324	WP_009953431.1	putative quinol monooxygenase	-
LK403_RS21290 (LK403_21290)	4601620..4602048	-	429	Protein_4223	hypothetical protein	-
LK403_RS21295 (LK403_21295)	4602201..4602545	+	345	WP_036458317.1	hypothetical protein	-
LK403_RS21300 (LK403_21300)	4602619..4603002	-	384	WP_196233639.1	hypothetical protein	-
LK403_RS21305 (LK403_21305)	4603102..4603500	-	399	WP_009953435.1	VOC family protein	-
LK403_RS21310 (LK403_21310)	4603579..4604130	-	552	WP_038535279.1	VOC family protein	-
LK403_RS21315 (LK403_21315)	4604188..4604559	-	372	WP_036458315.1	type II toxin-antitoxin system VapC family toxin	Toxin
LK403_RS21320 (LK403_21320)	4604546..4604752	-	207	WP_036458377.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LK403_RS21325 (LK403_21325)	4604991..4605653	+	663	WP_036458309.1	methyltransferase domain-containing protein	-
LK403_RS21330 (LK403_21330)	4605733..4606806	-	1074	WP_036458307.1	redox-regulated ATPase YchF	-
LK403_RS21335 (LK403_21335)	4606948..4608093	+	1146	WP_196233638.1	hypothetical protein	-
LK403_RS21340 (LK403_21340)	4608139..4609137	-	999	WP_008254378.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13905.22 Da
• Isoelectric Point: 6.9118

>T222289 WP_036458315.1 NZ_CP085945:c4604559-4604188 [Mycobacterium intracellulare]
VILVDTSIWIDHLHVAEHRLVGRLTNDEIGCHQMVIEELALGMIKQRDDVLRLLSHLREFPLLTHAEILHLVDRRRLWGR
GLSAIDAHLLGSVAVVDGARLWTRDKKLKAACREVGVAIVDES

Nucleotide

• Length: 372 bp

>T222289 NZ_CP117750:c3061666-3061256 [Enterobacter hormaechei]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGTGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCTGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7220.29 Da
• Isoelectric Point: 11.9169

>AT222289 WP_036458377.1 NZ_CP085945:c4604752-4604546 [Mycobacterium intracellulare]
MRTTVTIDDALLAQAAELTGVAESAALLRQGLQTLIRVESARRLAALGGTDPKASAAPRRRPPTRDPR

Nucleotide

• Length: 207 bp

>AT222289 NZ_CP117750:c3061913-3061647 [Enterobacter hormaechei]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA