Detailed information of TA system TA222288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3495324..3495988
• Replicon: chromosome
• Accession: NZ_CP085945
• Organism: Mycobacterium intracellulare strain FDAARGOS_1564

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: H8IUS5
• Locus tag: LK403_RS16315
• Protein ID: WP_014379897.1
• Coordinates: 3495324..3495731 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LK403_RS16320
• Protein ID: WP_009954496.1
• Coordinates: 3495728..3495988 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK403_RS16305 (LK403_16305)	3490419..3491984	+	1566	WP_014379899.1	serine/threonine-protein kinase	-
LK403_RS16310 (LK403_16310)	3492045..3495146	-	3102	WP_014379898.1	DEAD/DEAH box helicase	-
LK403_RS16315 (LK403_16315)	3495324..3495731	-	408	WP_014379897.1	type II toxin-antitoxin system VapC family toxin	Toxin
LK403_RS16320 (LK403_16320)	3495728..3495988	-	261	WP_009954496.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LK403_RS16325 (LK403_16325)	3496161..3496367	-	207	WP_225331375.1	hypothetical protein	-
LK403_RS16330 (LK403_16330)	3496431..3496883	-	453	WP_014379896.1	VOC family protein	-
LK403_RS16335 (LK403_16335)	3497043..3498311	+	1269	WP_095562638.1	cytochrome P450	-
LK403_RS16340 (LK403_16340)	3498316..3498912	-	597	WP_095562637.1	TetR/AcrR family transcriptional regulator	-
LK403_RS16345 (LK403_16345)	3498982..3499473	+	492	WP_095562636.1	DUF1707 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-66)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14307.31 Da
• Isoelectric Point: 4.2772

>T222288 WP_014379897.1 NZ_CP085945:c3495731-3495324 [Mycobacterium intracellulare]
MSNPQVAGVLDTSVFIATESGRPLDAALIPDQVGTTVVTLAELHVGVLAASTSDIRAQRLATLESIADIEALPIDDDAAR
MWARLRIHLAETGQRVRINDLWIAAIAASRGLPVVTQDDDFAVLDGAANLTVIRV

Nucleotide

• Length: 408 bp

>T222288 NZ_CP117750:2833174-2833473 [Enterobacter hormaechei]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATCCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9526.85 Da
• Isoelectric Point: 9.1203

>AT222288 WP_009954496.1 NZ_CP085945:c3495988-3495728 [Mycobacterium intracellulare]
MSEVASRELRNDTAGVLRRVRDGEDVTITVSGRPVAVLTAVVPQRRRWLSKTELLSRLPRVQADPGLREDLHALAGDTTE
ELGPIR

Nucleotide

• Length: 261 bp

>AT222288 NZ_CP117750:2833484-2833849 [Enterobacter hormaechei]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCATTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTGGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Toxin

• AlphaFold DB: A0A7R7MTD0