Detailed information of TA system TA222287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/ParD(antitoxin)
• Location: 3179841..3180833
• Replicon: chromosome
• Accession: NZ_CP085945
• Organism: Mycobacterium intracellulare strain FDAARGOS_1564

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: LK403_RS14970
• Protein ID: WP_009956379.1
• Coordinates: 3179841..3180419 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: A0A7I7TS83
• Locus tag: LK403_RS14975
• Protein ID: WP_009956380.1
• Coordinates: 3180444..3180833 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK403_RS14945 (LK403_14945)	3175799..3176770	-	972	WP_029385267.1	sigma-70 family RNA polymerase sigma factor	-
LK403_RS14950 (LK403_14950)	3176936..3177286	+	351	WP_009956374.1	DoxX family protein	-
LK403_RS14955 (LK403_14955)	3177298..3178098	-	801	WP_009956375.1	nuclear transport factor 2 family protein	-
LK403_RS14960 (LK403_14960)	3178098..3178658	-	561	WP_029385272.1	pyrazinamidase PncA	-
LK403_RS14965 (LK403_14965)	3178754..3179821	+	1068	WP_036397470.1	DoxX family protein	-
LK403_RS14970 (LK403_14970)	3179841..3180419	-	579	WP_009956379.1	zeta toxin family protein	Toxin
LK403_RS14975 (LK403_14975)	3180444..3180833	-	390	WP_009956380.1	hypothetical protein	Antitoxin
LK403_RS14980 (LK403_14980)	3180891..3181178	-	288	WP_225332075.1	hypothetical protein	-
LK403_RS14985 (LK403_14985)	3181206..3182165	-	960	WP_225331100.1	SAM-dependent methyltransferase	-
LK403_RS14990 (LK403_14990)	3182201..3182812	+	612	WP_009956383.1	TetR/AcrR family transcriptional regulator	-
LK403_RS14995 (LK403_14995)	3182836..3183381	-	546	WP_044059213.1	gluconokinase	-
LK403_RS15000 (LK403_15000)	3183378..3185381	-	2004	WP_009956386.1	phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-136)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 20962.05 Da
• Isoelectric Point: 11.0233

>T222287 WP_009956379.1 NZ_CP085945:c3180419-3179841 [Mycobacterium intracellulare]
MQRLDLVVGPNGAGKSTFIAFTLAPLLPGSPVVNADEIARQRWPGEAAGRAYDAAQIAAATRAKLIDLGRSFIAETVFSH
PSKLDLVRTAHRAGFTAVLHAMLIPEDLAVERVRHRVRAGGHDVPETKVRERHRRLWTPVTDAIALSDVATVYDSGRLRG
PRIVARLSGGMTIGSPDWPAWAPEILRSRWPD

Nucleotide

• Length: 579 bp

>T222287 NZ_CP117750:c2008858-2008755 [Enterobacter hormaechei]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13512.89 Da
• Isoelectric Point: 5.1828

>AT222287 WP_009956380.1 NZ_CP085945:c3180833-3180444 [Mycobacterium intracellulare]
MADAPDRVTRVASDLMDSAASEGARQSRSAKQQLDHWARVGRAVSSQHTASRRRVEAALAGQLATGELTVEEGVVFNAEI
SAAIEESLVHADYGATLAGHGVTTVALNEDGDIVEHRPDGAAVVLARGR

Nucleotide

• Length: 390 bp

>AT222287 NZ_CP117750:2008742-2008951 [Enterobacter hormaechei]
TTTTCAAGCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTA
AAAGTGGTCTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Structures

Antitoxin

• AlphaFold DB: A0A7I7TS83