Detailed information of TA system TA222281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 15730..16337
• Replicon: plasmid p4
• Accession: NZ_CP085910
• Organism: Enterococcus faecium strain SC1762-D

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: LLE91_RS16220
• Protein ID: WP_002321032.1
• Coordinates: 15987..16337 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: LLE91_RS16215
• Protein ID: WP_002287514.1
• Coordinates: 15730..15993 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE91_RS16195 (LLE91_16195)	11109..13877	-	2769	WP_016173094.1	sigma-54-dependent transcriptional regulator	-
LLE91_RS16200 (LLE91_16200)	14025..14624	-	600	WP_265582737.1	alpha/beta hydrolase	-
LLE91_RS16205 (LLE91_16205)	14694..15374	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
LLE91_RS16210 (LLE91_16210)	15412..15576	-	165	WP_164450253.1	hypothetical protein	-
LLE91_RS16215 (LLE91_16215)	15730..15993	+	264	WP_002287514.1	hypothetical protein	Antitoxin
LLE91_RS16220 (LLE91_16220)	15987..16337	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LLE91_RS16225 (LLE91_16225)	16361..16975	-	615	WP_010729795.1	recombinase family protein	-
LLE91_RS16230 (LLE91_16230)	17289..17711	+	423	WP_002288787.1	hypothetical protein	-
LLE91_RS16235 (LLE91_16235)	17721..17924	+	204	WP_002299573.1	hypothetical protein	-
LLE91_RS16240 (LLE91_16240)	18135..18719	+	585	WP_002299575.1	recombinase family protein	-
LLE91_RS16245 (LLE91_16245)	19090..20415	+	1326	WP_002303115.1	Y-family DNA polymerase	-
LLE91_RS16250 (LLE91_16250)	20408..20758	+	351	WP_002303114.1	hypothetical protein	-
LLE91_RS16255 (LLE91_16255)	20755..20934	+	180	WP_001021554.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..23177	23177
	-	inside	IScluster/Tn	-	-	14694..18719	4025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T222281 WP_002321032.1 NZ_CP085910:15987-16337 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T222281 NZ_CP117749:c5434030-5433731 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT222281 WP_002287514.1 NZ_CP085910:15730-15993 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT222281 NZ_CP117749:c5433734-5433444 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0