222280

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 74740..75877 Replicon plasmid pSC1762-D-vanA
Accession NZ_CP085908
Organism Enterococcus faecium strain SC1762-D

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag LLE91_RS15355 Protein ID WP_001284311.1
Coordinates 74740..75603 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag LLE91_RS15360 Protein ID WP_000301765.1
Coordinates 75605..75877 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLE91_RS15330 (LLE91_15330) 70168..70419 + 252 Protein_80 GNAT family N-acetyltransferase -
LLE91_RS15335 (LLE91_15335) 70512..71306 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
LLE91_RS15340 (LLE91_15340) 71969..73288 - 1320 WP_000202380.1 IS1380-like element IS1678 family transposase -
LLE91_RS15345 (LLE91_15345) 73532..73615 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
LLE91_RS15350 (LLE91_15350) 73740..74477 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LLE91_RS15355 (LLE91_15355) 74740..75603 - 864 WP_001284311.1 zeta toxin family protein Toxin
LLE91_RS15360 (LLE91_15360) 75605..75877 - 273 WP_000301765.1 antitoxin Antitoxin
LLE91_RS15365 (LLE91_15365) 75894..76109 - 216 WP_001835296.1 peptide-binding protein -
LLE91_RS15370 (LLE91_15370) 76201..76305 - 105 Protein_88 chromosome partitioning protein ParA -
LLE91_RS15375 (LLE91_15375) 76359..77039 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
LLE91_RS15380 (LLE91_15380) 77240..77824 + 585 WP_002347505.1 hypothetical protein -
LLE91_RS15385 (LLE91_15385) 77835..78080 + 246 WP_002347506.1 hypothetical protein -
LLE91_RS15390 (LLE91_15390) 78086..78808 + 723 WP_222036300.1 hypothetical protein -
LLE91_RS15395 (LLE91_15395) 78938..79681 + 744 WP_016922485.1 hypothetical protein -
LLE91_RS15400 (LLE91_15400) 79687..80052 + 366 WP_002347509.1 hypothetical protein -
LLE91_RS15405 (LLE91_15405) 80068..80598 + 531 WP_002347720.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / aph(3')-III / erm(B) - 1..134985 134985
- inside IScluster/Tn aph(3')-III / erm(B) - 61595..77045 15450


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T222280 WP_001284311.1 NZ_CP085908:c75603-74740 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T222280 NZ_CP085908:c75603-74740 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT222280 WP_000301765.1 NZ_CP085908:c75877-75605 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT222280 NZ_CP085908:c75877-75605 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References