Detailed information of TA system TA222278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 141596..142213
• Replicon: plasmid p1
• Accession: NZ_CP085907
• Organism: Enterococcus faecium strain SC1762-D

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LLE91_RS14415
• Protein ID: WP_224451579.1
• Coordinates: 142061..142213 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: LLE91_RS14410
• Protein ID: WP_002305052.1
• Coordinates: 141596..142027 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LLE91_RS14395 (LLE91_14395)	137411..138838	-	1428	WP_002361237.1	glycoside hydrolase family 32 protein	-
LLE91_RS14400 (LLE91_14400)	138892..140166	-	1275	WP_002323715.1	MFS transporter	-
LLE91_RS14405 (LLE91_14405)	140469..141422	+	954	WP_002287760.1	IS30 family transposase	-
LLE91_RS14410 (LLE91_14410)	141596..142027	-	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LLE91_RS14415 (LLE91_14415)	142061..142213	-	153	WP_224451579.1	type II toxin-antitoxin system HicA family toxin	Toxin
LLE91_RS14420 (LLE91_14420)	142307..142486	-	180	Protein_152	hypothetical protein	-
LLE91_RS14425 (LLE91_14425)	142502..142702	-	201	WP_002330566.1	hypothetical protein	-
LLE91_RS14430 (LLE91_14430)	142867..143574	-	708	WP_086325405.1	hypothetical protein	-
LLE91_RS14435 (LLE91_14435)	143567..144004	-	438	WP_002296237.1	hypothetical protein	-
LLE91_RS14440 (LLE91_14440)	144019..144270	-	252	WP_002296238.1	hypothetical protein	-
LLE91_RS14445 (LLE91_14445)	144270..144476	-	207	WP_002324499.1	hypothetical protein	-
LLE91_RS14450 (LLE91_14450)	144924..145124	-	201	WP_227005125.1	hypothetical protein	-
LLE91_RS14455 (LLE91_14455)	145316..145460	+	145	Protein_159	putative holin-like toxin	-
LLE91_RS14460 (LLE91_14460)	145619..145876	-	258	WP_002295625.1	hypothetical protein	-
LLE91_RS14465 (LLE91_14465)	145898..146383	-	486	Protein_161	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-aph(2'')	-	1..224638	224638
	-	flank	IS/Tn	-	-	140469..141422	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5815.65 Da
• Isoelectric Point: 10.3560

>T222278 WP_224451579.1 NZ_CP085907:c142213-142061 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 153 bp

>T222278 NZ_CP117749:c4539523-4538768 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATGTCCGACCGCTCCAGGGGCGGCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGTTGGGGG
TCGCGGTTTGGCCGTCGTCACGAGCCAAGCCTGTTCTACGCCGCGTTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGTGTCCTGTGGAGTGGCATGGCAGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGGAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCTGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT222278 WP_002305052.1 NZ_CP085907:c142027-141596 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT222278 NZ_CP117749:c4540026-4539658 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCTGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGGGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8