Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 497951..498522 Replicon chromosome
Accession NZ_CP085906
Organism Enterococcus faecium strain SC1762-D

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag LLE91_RS02385 Protein ID WP_002286801.1
Coordinates 498181..498522 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LLE91_RS02380 Protein ID WP_002323011.1
Coordinates 497951..498181 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLE91_RS02355 (LLE91_02355) 493430..494758 + 1329 WP_044383738.1 FAD-dependent oxidoreductase -
LLE91_RS02360 (LLE91_02360) 494780..495406 + 627 WP_002286816.1 cysteine hydrolase -
LLE91_RS02365 (LLE91_02365) 495589..496170 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
LLE91_RS02370 (LLE91_02370) 496537..497112 + 576 WP_002331662.1 SOS response-associated peptidase family protein -
LLE91_RS02375 (LLE91_02375) 497317..497655 - 339 WP_044383733.1 hypothetical protein -
LLE91_RS02380 (LLE91_02380) 497951..498181 + 231 WP_002323011.1 hypothetical protein Antitoxin
LLE91_RS02385 (LLE91_02385) 498181..498522 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LLE91_RS02390 (LLE91_02390) 499372..499557 + 186 WP_002304207.1 hypothetical protein -
LLE91_RS02395 (LLE91_02395) 499827..500989 + 1163 WP_086956687.1 IS3 family transposase -
LLE91_RS02400 (LLE91_02400) 501120..501362 + 243 Protein_475 LPXTG cell wall anchor domain-containing protein -
LLE91_RS02405 (LLE91_02405) 501436..502332 + 897 WP_002352497.1 class C sortase -
LLE91_RS02410 (LLE91_02410) 502513..503187 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T222276 WP_002286801.1 NZ_CP085906:498181-498522 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T222276 NZ_CP117749:2699936-2700511 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT222276 WP_002323011.1 NZ_CP085906:497951-498181 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT222276 NZ_CP117749:2699469-2699939 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References