222275

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 326194..326765 Replicon chromosome
Accession NZ_CP085906
Organism Enterococcus faecium strain SC1762-D

Toxin (Protein)


Gene name mazF Uniprot ID A0A2W4HYX0
Locus tag LLE91_RS01585 Protein ID WP_002315988.1
Coordinates 326424..326765 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag LLE91_RS01580 Protein ID WP_025477367.1
Coordinates 326194..326424 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LLE91_RS01565 (LLE91_01565) 322462..323901 - 1440 Protein_312 helix-turn-helix domain-containing protein -
LLE91_RS01570 (LLE91_01570) 324045..324434 + 390 WP_227889183.1 MmcQ/YjbR family DNA-binding protein -
LLE91_RS01575 (LLE91_01575) 324476..325939 + 1464 WP_073466279.1 IS1182 family transposase -
LLE91_RS01580 (LLE91_01580) 326194..326424 + 231 WP_025477367.1 hypothetical protein Antitoxin
LLE91_RS01585 (LLE91_01585) 326424..326765 + 342 WP_002315988.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LLE91_RS01590 (LLE91_01590) 327460..329541 + 2082 WP_002315986.1 PTS sugar transporter subunit IIA -
LLE91_RS01595 (LLE91_01595) 329577..329879 + 303 WP_002292534.1 PTS sugar transporter subunit IIB -
LLE91_RS01600 (LLE91_01600) 329905..331194 + 1290 WP_002315985.1 PTS sugar transporter subunit IIC -
LLE91_RS01605 (LLE91_01605) 331213..331620 + 408 WP_002315984.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 324494..325939 1445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13330.69 Da        Isoelectric Point: 9.9258

>T222275 WP_002315988.1 NZ_CP085906:326424-326765 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T222275 NZ_CP117749:1895245-1895832 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT222275 WP_025477367.1 NZ_CP085906:326194-326424 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp

>AT222275 NZ_CP117749:1895899-1896753 [Pseudomonas aeruginosa]
GTGCCGGACGAGATGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCCGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGATGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W4HYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References