Detailed information of TA system TA222273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 15735..16342
• Replicon: plasmid p4
• Accession: NZ_CP085898
• Organism: Enterococcus faecium strain SC1762

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: LK894_RS16195
• Protein ID: WP_002321032.1
• Coordinates: 15992..16342 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: LK894_RS16190
• Protein ID: WP_002287514.1
• Coordinates: 15735..15998 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LK894_RS16170 (LK894_16185)	11114..13882	-	2769	WP_016173094.1	sigma-54-dependent transcriptional regulator	-
LK894_RS16175 (LK894_16190)	14030..14629	-	600	WP_265582737.1	alpha/beta hydrolase	-
LK894_RS16180 (LK894_16195)	14699..15379	-	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
LK894_RS16185 (LK894_16200)	15417..15581	-	165	WP_164450253.1	hypothetical protein	-
LK894_RS16190 (LK894_16205)	15735..15998	+	264	WP_002287514.1	hypothetical protein	Antitoxin
LK894_RS16195 (LK894_16210)	15992..16342	+	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LK894_RS16200 (LK894_16215)	16366..16980	-	615	WP_010729795.1	recombinase family protein	-
LK894_RS16205 (LK894_16220)	17294..17716	+	423	WP_002288787.1	hypothetical protein	-
LK894_RS16210 (LK894_16225)	17726..17929	+	204	WP_002299573.1	hypothetical protein	-
LK894_RS16215 (LK894_16230)	18140..18724	+	585	WP_002299575.1	recombinase family protein	-
LK894_RS16220 (LK894_16235)	19095..20420	+	1326	WP_002303115.1	Y-family DNA polymerase	-
LK894_RS16225 (LK894_16240)	20413..20763	+	351	WP_002303114.1	hypothetical protein	-
LK894_RS16230 (LK894_16245)	20760..20939	+	180	WP_001021554.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..23182	23182
	-	inside	IScluster/Tn	-	-	14699..18724	4025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T222273 WP_002321032.1 NZ_CP085898:15992-16342 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T222273 NZ_CP117746:c318351-317908 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT222273 WP_002287514.1 NZ_CP085898:15735-15998 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT222273 NZ_CP117746:c318578-318348 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0