Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 73923..75060 Replicon plasmid pSC1762-vanA
Accession NZ_CP085896
Organism Enterococcus faecium strain SC1762

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag LK894_RS15330 Protein ID WP_001284311.1
Coordinates 73923..74786 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag LK894_RS15335 Protein ID WP_000301765.1
Coordinates 74788..75060 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK894_RS15305 (LK894_15320) 69351..69602 + 252 Protein_80 GNAT family N-acetyltransferase -
LK894_RS15310 (LK894_15325) 69695..70489 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
LK894_RS15315 (LK894_15330) 71152..72471 - 1320 WP_000202380.1 IS1380-like element IS1678 family transposase -
LK894_RS15320 (LK894_15335) 72715..72798 + 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
LK894_RS15325 (LK894_15340) 72923..73660 + 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LK894_RS15330 (LK894_15345) 73923..74786 - 864 WP_001284311.1 zeta toxin family protein Toxin
LK894_RS15335 (LK894_15350) 74788..75060 - 273 WP_000301765.1 antitoxin Antitoxin
LK894_RS15340 (LK894_15355) 75077..75292 - 216 WP_001835296.1 peptide-binding protein -
LK894_RS15345 (LK894_15360) 75384..75488 - 105 Protein_88 chromosome partitioning protein ParA -
LK894_RS15350 (LK894_15365) 75542..76222 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
LK894_RS15355 (LK894_15370) 76423..77007 + 585 WP_002347505.1 hypothetical protein -
LK894_RS15360 (LK894_15375) 77018..77263 + 246 WP_002347506.1 hypothetical protein -
LK894_RS15365 (LK894_15380) 77269..77991 + 723 WP_222036300.1 hypothetical protein -
LK894_RS15370 (LK894_15385) 78121..78864 + 744 WP_016922485.1 hypothetical protein -
LK894_RS15375 (LK894_15390) 78870..79235 + 366 WP_002347509.1 hypothetical protein -
LK894_RS15380 (LK894_15395) 79251..79781 + 531 WP_002347720.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / aph(3')-III / erm(B) - 1..134168 134168
- inside IScluster/Tn aph(3')-III / erm(B) - 60778..76228 15450


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T222272 WP_001284311.1 NZ_CP085896:c74786-73923 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T222272 NZ_CP117746:181086-181556 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACTCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT222272 WP_000301765.1 NZ_CP085896:c75060-74788 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT222272 NZ_CP117746:180646-181089 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCCAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACTTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References