Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/HicB-HicA |
| Location | 141595..142212 | Replicon | plasmid p1 |
| Accession | NZ_CP085895 | ||
| Organism | Enterococcus faecium strain SC1762 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | - |
| Locus tag | LK894_RS14390 | Protein ID | WP_224451579.1 |
| Coordinates | 142060..142212 (-) | Length | 51 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A829EWE8 |
| Locus tag | LK894_RS14385 | Protein ID | WP_002305052.1 |
| Coordinates | 141595..142026 (-) | Length | 144 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LK894_RS14370 (LK894_14390) | 137410..138837 | - | 1428 | WP_002361237.1 | glycoside hydrolase family 32 protein | - |
| LK894_RS14375 (LK894_14395) | 138891..140165 | - | 1275 | WP_002323715.1 | MFS transporter | - |
| LK894_RS14380 (LK894_14400) | 140468..141421 | + | 954 | WP_002287760.1 | IS30 family transposase | - |
| LK894_RS14385 (LK894_14405) | 141595..142026 | - | 432 | WP_002305052.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| LK894_RS14390 (LK894_14410) | 142060..142212 | - | 153 | WP_224451579.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| LK894_RS14395 (LK894_14415) | 142306..142485 | - | 180 | Protein_152 | hypothetical protein | - |
| LK894_RS14400 (LK894_14420) | 142501..142701 | - | 201 | WP_002330566.1 | hypothetical protein | - |
| LK894_RS14405 (LK894_14425) | 142866..143573 | - | 708 | WP_086325405.1 | hypothetical protein | - |
| LK894_RS14410 (LK894_14430) | 143566..144003 | - | 438 | WP_002296237.1 | hypothetical protein | - |
| LK894_RS14415 (LK894_14435) | 144018..144269 | - | 252 | WP_002296238.1 | hypothetical protein | - |
| LK894_RS14420 (LK894_14440) | 144269..144475 | - | 207 | WP_002324499.1 | hypothetical protein | - |
| LK894_RS14425 (LK894_14445) | 144923..145123 | - | 201 | WP_227005125.1 | hypothetical protein | - |
| LK894_RS14430 (LK894_14450) | 145315..145459 | + | 145 | Protein_159 | putative holin-like toxin | - |
| LK894_RS14435 (LK894_14455) | 145618..145875 | - | 258 | WP_002295625.1 | hypothetical protein | - |
| LK894_RS14440 (LK894_14460) | 145897..146382 | - | 486 | Protein_161 | single-stranded DNA-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | aac(6')-aph(2'') | - | 1..224636 | 224636 | |
| - | flank | IS/Tn | - | - | 140468..141421 | 953 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 51 a.a. Molecular weight: 5815.65 Da Isoelectric Point: 10.3560
>T222270 WP_224451579.1 NZ_CP085895:c142212-142060 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK
Download Length: 153 bp
>T222270 NZ_CP117745:c5330773-5330390 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA
Antitoxin
Download Length: 144 a.a. Molecular weight: 15811.92 Da Isoelectric Point: 4.1638
>AT222270 WP_002305052.1 NZ_CP085895:c142026-141595 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN
Download Length: 432 bp
>AT222270 NZ_CP117745:c5331015-5330773 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|