222269

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 50862..51469 Replicon plasmid p1
Accession NZ_CP085895
Organism Enterococcus faecium strain SC1762

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag LK894_RS13890 Protein ID WP_002321032.1
Coordinates 51119..51469 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag LK894_RS13885 Protein ID WP_002287514.1
Coordinates 50862..51125 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK894_RS13840 (LK894_13860) 45909..46808 + 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LK894_RS13845 (LK894_13865) 46811..47296 + 486 WP_002296241.1 single-stranded DNA-binding protein -
LK894_RS13850 (LK894_13870) 47662..47922 + 261 WP_002296240.1 hypothetical protein -
LK894_RS13855 (LK894_13875) 48363..48569 + 207 WP_002296239.1 hypothetical protein -
LK894_RS13860 (LK894_13880) 48569..48820 + 252 WP_002287517.1 hypothetical protein -
LK894_RS13865 (LK894_13885) 48836..49273 + 438 WP_002305049.1 hypothetical protein -
LK894_RS13870 (LK894_13890) 49266..49961 + 696 WP_002305050.1 hypothetical protein -
LK894_RS13875 (LK894_13895) 50126..50326 + 201 WP_002324500.1 hypothetical protein -
LK894_RS13880 (LK894_13900) 50342..50521 + 180 WP_002305761.1 hypothetical protein -
LK894_RS13885 (LK894_13905) 50862..51125 + 264 WP_002287514.1 hypothetical protein Antitoxin
LK894_RS13890 (LK894_13910) 51119..51469 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LK894_RS13895 (LK894_13915) 51493..52107 - 615 WP_010729795.1 recombinase family protein -
LK894_RS13900 (LK894_13920) 52421..52843 + 423 WP_002288787.1 hypothetical protein -
LK894_RS13905 (LK894_13925) 52853..53056 + 204 WP_002299573.1 hypothetical protein -
LK894_RS13910 (LK894_13930) 53267..53851 + 585 WP_002299575.1 recombinase family protein -
LK894_RS13915 (LK894_13935) 54040..54720 - 681 WP_010861579.1 IS6-like element IS1216 family transposase -
LK894_RS13920 (LK894_13940) 54774..55484 - 711 WP_010729797.1 GntR family transcriptional regulator -
LK894_RS13925 (LK894_13945) 55468..56202 - 735 WP_010729798.1 GntR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..224636 224636


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T222269 WP_002321032.1 NZ_CP085895:51119-51469 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T222269 NZ_CP117745:c5006967-5006092 [Klebsiella pneumoniae]
ATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATTTGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGA
TTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGGTGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGG
ATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAGGCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAG
ATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAAGATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACC
ACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTTTTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCT
ACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCATCCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAG
GAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTATTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCC
TCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAATTCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGA
CAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCCTGTATCACACAACAGGATGTCGATTTTTTACTGTCATTTAAA
CGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCGTATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATAT
TCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAGATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT222269 WP_002287514.1 NZ_CP085895:50862-51125 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT222269 NZ_CP117745:c5007773-5007015 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References