Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 496550..497121 Replicon chromosome
Accession NZ_CP085894
Organism Enterococcus faecium strain SC1762

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag LK894_RS02380 Protein ID WP_002286801.1
Coordinates 496780..497121 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag LK894_RS02375 Protein ID WP_002323011.1
Coordinates 496550..496780 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK894_RS02350 (LK894_02350) 492029..493357 + 1329 WP_044383738.1 FAD-dependent oxidoreductase -
LK894_RS02355 (LK894_02355) 493379..494005 + 627 WP_002286816.1 cysteine hydrolase -
LK894_RS02360 (LK894_02360) 494188..494769 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
LK894_RS02365 (LK894_02365) 495136..495711 + 576 WP_002331662.1 SOS response-associated peptidase family protein -
LK894_RS02370 (LK894_02370) 495916..496254 - 339 WP_044383733.1 hypothetical protein -
LK894_RS02375 (LK894_02375) 496550..496780 + 231 WP_002323011.1 hypothetical protein Antitoxin
LK894_RS02380 (LK894_02380) 496780..497121 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LK894_RS02385 (LK894_02385) 497971..498156 + 186 WP_002304207.1 hypothetical protein -
LK894_RS02390 (LK894_02390) 498426..499588 + 1163 WP_086956687.1 IS3 family transposase -
LK894_RS02395 (LK894_02395) 499719..499961 + 243 Protein_474 LPXTG cell wall anchor domain-containing protein -
LK894_RS02400 (LK894_02400) 500035..500931 + 897 WP_002352497.1 class C sortase -
LK894_RS02405 (LK894_02405) 501112..501786 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T222268 WP_002286801.1 NZ_CP085894:496780-497121 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T222268 NZ_CP117745:c4846637-4846353 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT222268 WP_002323011.1 NZ_CP085894:496550-496780 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT222268 NZ_CP117745:c4846869-4846627 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References