222267

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 326315..326886 Replicon chromosome
Accession NZ_CP085894
Organism Enterococcus faecium strain SC1762

Toxin (Protein)


Gene name mazF Uniprot ID A0A2W4HYX0
Locus tag LK894_RS01585 Protein ID WP_002315988.1
Coordinates 326545..326886 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag LK894_RS01580 Protein ID WP_025477367.1
Coordinates 326315..326545 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK894_RS01565 (LK894_01565) 322583..324022 - 1440 Protein_312 helix-turn-helix domain-containing protein -
LK894_RS01570 (LK894_01570) 324166..324555 + 390 WP_227889183.1 MmcQ/YjbR family DNA-binding protein -
LK894_RS01575 (LK894_01575) 324597..326060 + 1464 WP_073466279.1 IS1182 family transposase -
LK894_RS01580 (LK894_01580) 326315..326545 + 231 WP_025477367.1 hypothetical protein Antitoxin
LK894_RS01585 (LK894_01585) 326545..326886 + 342 WP_002315988.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LK894_RS01590 (LK894_01590) 327581..329662 + 2082 WP_002315986.1 PTS sugar transporter subunit IIA -
LK894_RS01595 (LK894_01595) 329698..330000 + 303 WP_002292534.1 PTS sugar transporter subunit IIB -
LK894_RS01600 (LK894_01600) 330026..331315 + 1290 WP_002315985.1 PTS sugar transporter subunit IIC -
LK894_RS01605 (LK894_01605) 331334..331741 + 408 WP_002315984.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 324615..326060 1445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13330.69 Da        Isoelectric Point: 9.9258

>T222267 WP_002315988.1 NZ_CP085894:326545-326886 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T222267 NZ_CP117745:c4736899-4736366 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT222267 WP_025477367.1 NZ_CP085894:326315-326545 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp

>AT222267 NZ_CP117745:c4737176-4736910 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W4HYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References