Detailed information of TA system TA222262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 10325..11235
• Replicon: plasmid pFK8695-rmpA
• Accession: NZ_CP085891
• Organism: Klebsiella pneumoniae strain 8695

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LKW25_RS28105
• Protein ID: WP_004026354.1
• Coordinates: 10325..10795 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LKW25_RS28110
• Protein ID: WP_004026357.1
• Coordinates: 10792..11235 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKW25_RS28080 (LKW25_28080)	5737..6135	-	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-
LKW25_RS28085 (LKW25_28085)	6472..6966	-	495	WP_004212794.1	thermonuclease family protein	-
LKW25_RS28090 (LKW25_28090)	7144..7410	+	267	WP_223175074.1	DUF1173 family protein	-
LKW25_RS28095 (LKW25_28095)	7443..8093	+	651	WP_068893702.1	DUF1173 family protein	-
LKW25_RS28105 (LKW25_28105)	10325..10795	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LKW25_RS28110 (LKW25_28110)	10792..11235	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LKW25_RS28115 (LKW25_28115)	11336..11839	-	504	Protein_13	DUF4113 domain-containing protein	-
LKW25_RS28120 (LKW25_28120)	12045..13025	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
LKW25_RS28125 (LKW25_28125)	13091..14398	+	1308	Protein_15	FepA family TonB-dependent siderophore receptor	-
LKW25_RS28130 (LKW25_28130)	14460..14681	+	222	WP_004213615.1	hypothetical protein	-
LKW25_RS28135 (LKW25_28135)	14824..15726	-	903	WP_004213613.1	DMT family inner membrane transporter PEG344	-
LKW25_RS28140 (LKW25_28140)	15799..16200	-	402	WP_214588034.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	iroN / rmpA / rmpA	1..150052	150052
	-	inside	IScluster/Tn	-	-	4286..13025	8739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T222262 WP_004026354.1 NZ_CP085891:c10795-10325 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T222262 NZ_CP117745:1456140-1456556 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACCGACATCCGGGTGGCGCTGCACCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGCCACGCCATCGCGGCGGGCGCCGTCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCTGGACTGACACTGG
AAGACTGGGTTATTTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT222262 WP_004026357.1 NZ_CP085891:c11235-10792 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT222262 NZ_CP117745:1455913-1456143 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9