Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 131283..131926 Replicon plasmid pFK8695-KPC-33
Accession NZ_CP085890
Organism Klebsiella pneumoniae strain 8695

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LKW25_RS27970 Protein ID WP_001044770.1
Coordinates 131510..131926 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LKW25_RS27965 Protein ID WP_001261282.1
Coordinates 131283..131513 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKW25_RS27930 (126506) 126506..127285 - 780 WP_013214009.1 site-specific integrase -
LKW25_RS27935 (127469) 127469..128473 - 1005 WP_011977814.1 hypothetical protein -
LKW25_RS27940 (128503) 128503..128706 - 204 WP_011977813.1 hypothetical protein -
LKW25_RS27945 (128752) 128752..129273 - 522 WP_013214008.1 hypothetical protein -
LKW25_RS27950 (129331) 129331..129723 - 393 WP_011977811.1 hypothetical protein -
LKW25_RS27955 (129760) 129760..130704 - 945 WP_011977810.1 hypothetical protein -
LKW25_RS27960 (130865) 130865..131326 - 462 WP_014343465.1 hypothetical protein -
LKW25_RS27965 (131283) 131283..131513 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LKW25_RS27970 (131510) 131510..131926 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LKW25_RS27975 (132000) 132000..133562 + 1563 WP_004206609.1 AAA family ATPase -
LKW25_RS27980 (133547) 133547..134569 + 1023 WP_000361404.1 helicase UvrD -
LKW25_RS27985 (134825) 134825..135522 + 698 WP_223174630.1 IS1 family transposase -
LKW25_RS27990 (135551) 135551..135823 + 273 Protein_179 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-33 / blaSHV-12 / blaCTX-M-65 - 1..143980 143980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T222261 WP_001044770.1 NZ_CP085890:131510-131926 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T222261 NZ_CP117745:837783-838193 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT222261 WP_001261282.1 NZ_CP085890:131283-131513 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT222261 NZ_CP117745:837536-837802 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References