Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 878149..878803 Replicon chromosome
Accession NZ_CP085889
Organism Klebsiella pneumoniae strain 8695

Toxin (Protein)


Gene name cptA Uniprot ID R4YHX2
Locus tag LKW25_RS04430 Protein ID WP_004144731.1
Coordinates 878396..878803 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag LKW25_RS04425 Protein ID WP_002916312.1
Coordinates 878149..878415 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKW25_RS04400 (LKW25_04400) 873305..874738 - 1434 WP_002916322.1 6-phospho-beta-glucosidase BglA -
LKW25_RS04405 (LKW25_04405) 874857..875585 - 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
LKW25_RS04410 (LKW25_04410) 875635..875946 + 312 WP_002916319.1 N(4)-acetylcytidine aminohydrolase -
LKW25_RS04415 (LKW25_04415) 876110..876769 + 660 WP_002916317.1 hemolysin III family protein -
LKW25_RS04420 (LKW25_04420) 876920..877903 - 984 WP_002916313.1 tRNA-modifying protein YgfZ -
LKW25_RS04425 (LKW25_04425) 878149..878415 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
LKW25_RS04430 (LKW25_04430) 878396..878803 + 408 WP_004144731.1 protein YgfX Toxin
LKW25_RS04435 (LKW25_04435) 878810..879331 - 522 WP_002916308.1 flavodoxin FldB -
LKW25_RS04440 (LKW25_04440) 879432..880328 + 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
LKW25_RS04445 (LKW25_04445) 880351..881064 + 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LKW25_RS04450 (LKW25_04450) 881070..882803 + 1734 WP_004151783.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15936.69 Da        Isoelectric Point: 11.4778

>T222240 WP_004144731.1 NZ_CP085889:878396-878803 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 408 bp

>T222240 NZ_CP117730:c5846475-5846101 [Klebsiella grimontii]
ATGATTAAAGGCCCGGCGCTGTTTGATACTAATATTCTCATCGATTTATTCAGCGGTCGCGTTGAAGCGAAACAGGTTCT
GGAGGCTTATCCGCCACAGAATGCGATTAGCCTCATCACGTGGATGGAAGTAATGGTGGGGGCGAAAAAGTATCATCAGG
AACACCGCACGCGTATTGCACTGAGCGCGTTTAATATTATCGATATTTCACAGGATATTGCTGAACGAAGCGTCAATCTC
AGGAAGGAGTATGGGATGAAGCTGCCGGATGCCATCATACTGGCGACAGCGCAAATACATCGTTATGAACTGGTCACGCG
CAATACCAAAGATTTTTTCGGGATACCCGGAGTGATTACGCTTTATCATTTGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT222240 WP_002916312.1 NZ_CP085889:878149-878415 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT222240 NZ_CP117730:c5846711-5846472 [Klebsiella grimontii]
ATGATGACGGGAACGAATATGGGCAGAATACTACTCGACTTATCGGATGACGTTATTAAACGTCTTGAAGACCTCAAACA
GCAGCGCAATCTTCCTCGAGCGGAACTGTTACGCGAGGCGGTTGAACAGTACCTGGAAAGACAAGCTGACACCACTATTT
CCAATGCGCTGGGGATTTGGCAAGGCTGTGAGGAAGATGGAGTTGAATTCGAAAGGAAACTCCGCGAGGAGTGGCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378E4P6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References