Detailed information of TA system TA022224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2202899..2203602
• Replicon: chromosome
• Accession: NC_009565
• Organism: Mycobacterium tuberculosis F11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: TBFG_RS10210
• Protein ID: WP_003409778.1
• Coordinates: 2202899..2203228 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: TBFG_RS21360
• Protein ID: WP_003409780.1
• Coordinates: 2203225..2203602 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBFG_RS10190	2199282..2200352	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
TBFG_RS10195	2200349..2200864	+	516	WP_012054301.1	flavin reductase family protein	-
TBFG_RS10200	2200861..2201922	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
TBFG_RS10205	2201919..2202689	+	771	WP_003409775.1	SDR family oxidoreductase	-
TBFG_RS10210	2202899..2203228	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TBFG_RS21360	2203225..2203602	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
TBFG_RS10220	2203599..2204189	-	591	WP_003409784.1	SEC-C domain-containing protein	-
TBFG_RS10225	2204244..2205608	+	1365	WP_012054302.1	HNH endonuclease	-
TBFG_RS10230	2205763..2206215	-	453	WP_003899095.1	lipoprotein	-
TBFG_RS10235	2206279..2206680	+	402	WP_003409869.1	hypothetical protein	-
TBFG_RS10240	2206673..2206855	-	183	WP_003409870.1	hypothetical protein	-
TBFG_RS10245	2206969..2207319	-	351	WP_003899096.1	hypothetical protein	-
TBFG_RS10250	2207330..2208232	-	903	WP_003899097.1	hypothetical protein	-
TBFG_RS10255	2208253..2208444	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T22224 WP_003409778.1 NC_009565:c2203228-2202899 [Mycobacterium tuberculosis F11]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T22224 NC_009565:c2203228-2202899 [Mycobacterium tuberculosis F11]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT22224 WP_003409780.1 NC_009565:c2203602-2203225 [Mycobacterium tuberculosis F11]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT22224 NC_009565:c2203602-2203225 [Mycobacterium tuberculosis F11]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8