Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 77711..78347 Replicon plasmid pHN2
Accession NZ_CP085886
Organism Pseudomonas sp. HN2

Toxin (Protein)


Gene name hicA Uniprot ID -
Locus tag LIS66_RS27630 Protein ID WP_227974596.1
Coordinates 77711..77893 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LIS66_RS27635 Protein ID WP_227974597.1
Coordinates 77925..78347 (+) Length 141 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIS66_RS27605 (LIS66_27605) 72901..73914 + 1014 WP_227974591.1 hypothetical protein -
LIS66_RS27610 (LIS66_27610) 74688..75074 + 387 WP_227974592.1 hypothetical protein -
LIS66_RS27615 (LIS66_27615) 75287..75613 + 327 WP_227974593.1 hypothetical protein -
LIS66_RS27620 (LIS66_27620) 75661..77130 + 1470 WP_227974594.1 hypothetical protein -
LIS66_RS27625 (LIS66_27625) 77274..77609 + 336 WP_227974595.1 hypothetical protein -
LIS66_RS27630 (LIS66_27630) 77711..77893 + 183 WP_227974596.1 type II toxin-antitoxin system HicA family toxin Toxin
LIS66_RS27635 (LIS66_27635) 77925..78347 + 423 WP_227974597.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
LIS66_RS27640 (LIS66_27640) 78347..78754 + 408 WP_227974598.1 S24 family peptidase -
LIS66_RS27645 (LIS66_27645) 78738..79274 + 537 WP_227974599.1 hypothetical protein -
LIS66_RS27650 (LIS66_27650) 79294..79605 + 312 WP_227974600.1 hypothetical protein -
LIS66_RS27655 (LIS66_27655) 79620..80054 + 435 WP_227974601.1 hypothetical protein -
LIS66_RS27660 (LIS66_27660) 80140..80337 + 198 WP_227974602.1 hypothetical protein -
LIS66_RS27665 (LIS66_27665) 80517..80789 + 273 WP_227974603.1 hypothetical protein -
LIS66_RS27670 (LIS66_27670) 80825..81457 + 633 WP_227974604.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..81985 81985


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-58)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6868.01 Da        Isoelectric Point: 10.5498

>T222236 WP_227974596.1 NZ_CP085886:77711-77893 [Pseudomonas sp. HN2]
MKCSEFRRWLVAQGVEFKSAKGSHFKVYLNGKSTIFADHGSKEMHEGLRKTIIKQLGLKD

Download         Length: 183 bp

>T222236 NZ_CP117730:c1265045-1264827 [Klebsiella grimontii]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCCGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin


Download         Length: 141 a.a.        Molecular weight: 15369.81 Da        Isoelectric Point: 4.9408

>AT222236 WP_227974597.1 NZ_CP085886:77925-78347 [Pseudomonas sp. HN2]
MYQYPLELHTEPTGVWLSCPDVPEMNASGDTLGEAFAEALNGLESALSLYVEQRRKIPAASPAQDPALVLRLPALTVAKI
MLWNAMCDEGVSRAELARRLGVSRQMVDRLVDFLHASKIEQVERALGLLGRRLSLVLEAA

Download         Length: 423 bp

>AT222236 NZ_CP117730:c1265446-1265072 [Klebsiella grimontii]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATTACTCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGTGCGGATCTGCAGAAATGGCGCAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References