Detailed information of TA system TA222227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1747227..1747843
• Replicon: chromosome
• Accession: NZ_CP085885
• Organism: Pseudomonas sp. HN2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LIS66_RS07705
• Protein ID: WP_042607563.1
• Coordinates: 1747227..1747439 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LIS66_RS07710
• Protein ID: WP_227973925.1
• Coordinates: 1747439..1747843 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS66_RS07675 (LIS66_07675)	1743330..1743866	+	537	WP_064365457.1	DsbE family thiol:disulfide interchange protein	-
LIS66_RS07680 (LIS66_07680)	1743863..1744333	+	471	WP_064365456.1	cytochrome c-type biogenesis protein CcmH	-
LIS66_RS07685 (LIS66_07685)	1744330..1745529	+	1200	WP_042607559.1	c-type cytochrome biogenesis protein CcmI	-
LIS66_RS07690 (LIS66_07690)	1745555..1745959	+	405	WP_065617394.1	hypothetical protein	-
LIS66_RS07695 (LIS66_07695)	1746004..1746578	-	575	Protein_1517	PIN domain-containing protein	-
LIS66_RS07700 (LIS66_07700)	1746575..1747042	-	468	WP_227973924.1	helix-turn-helix domain-containing protein	-
LIS66_RS07705 (LIS66_07705)	1747227..1747439	+	213	WP_042607563.1	type II toxin-antitoxin system HicA family toxin	Toxin
LIS66_RS07710 (LIS66_07710)	1747439..1747843	+	405	WP_227973925.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LIS66_RS07715 (LIS66_07715)	1747956..1749155	-	1200	WP_227973926.1	MFS transporter	-
LIS66_RS07720 (LIS66_07720)	1749564..1750562	-	999	WP_123420176.1	sulfate ABC transporter substrate-binding protein	-
LIS66_RS07725 (LIS66_07725)	1750739..1751563	-	825	WP_064365449.1	ion transporter	-
LIS66_RS07730 (LIS66_07730)	1751585..1752499	-	915	WP_227973927.1	urea transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 7907.02 Da
• Isoelectric Point: 10.5973

>T222227 WP_042607563.1 NZ_CP085885:1747227-1747439 [Pseudomonas sp. HN2]
VQSRLLMKELEEAGWTLDRVAGSHHIFTHRYNPNTIAVPHPKKDLPLGTVTSIRRRAGLFSPPTRHEGDP

Nucleotide

• Length: 213 bp

>T222227 NZ_CP117724:c171782-171297 [Enterobacter asburiae]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14735.75 Da
• Isoelectric Point: 4.5789

>AT222227 WP_227973925.1 NZ_CP085885:1747439-1747843 [Pseudomonas sp. HN2]
MQYPICIEWGDENTAIGIQIPDIPGAVTAGDSFEEAYNAAVEVAHLMLQEIAAAGQAIPMPTSAAEHRSHPDFAGMGWGM
LELDISPYLGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT222227 NZ_CP117724:c172036-171770 [Enterobacter asburiae]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA