Detailed information of TA system TA222225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 183572..184088
• Replicon: chromosome
• Accession: NZ_CP085885
• Organism: Pseudomonas sp. HN2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LIS66_RS00790
• Protein ID: WP_042610152.1
• Coordinates: 183807..184088 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2U1R4X8
• Locus tag: LIS66_RS00785
• Protein ID: WP_041477418.1
• Coordinates: 183572..183817 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIS66_RS00765 (LIS66_00765)	179017..180555	+	1539	WP_116658917.1	MFS transporter	-
LIS66_RS00770 (LIS66_00770)	180584..181651	+	1068	WP_227973408.1	HlyD family secretion protein	-
LIS66_RS00775 (LIS66_00775)	181648..183216	+	1569	WP_227973409.1	efflux transporter outer membrane subunit	-
LIS66_RS00780 (LIS66_00780)	183295..183534	+	240	WP_064361654.1	DUF2789 domain-containing protein	-
LIS66_RS00785 (LIS66_00785)	183572..183817	+	246	WP_041477418.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LIS66_RS00790 (LIS66_00790)	183807..184088	+	282	WP_042610152.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LIS66_RS00795 (LIS66_00795)	184452..184880	+	429	WP_016772266.1	winged helix DNA-binding protein	-
LIS66_RS00800 (LIS66_00800)	184877..186940	+	2064	WP_227973410.1	FUSC family protein	-
LIS66_RS00805 (LIS66_00805)	186937..187146	+	210	WP_016772264.1	DUF1656 domain-containing protein	-
LIS66_RS00810 (LIS66_00810)	187143..188030	+	888	WP_227973411.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10814.71 Da
• Isoelectric Point: 10.5399

>T222225 WP_042610152.1 NZ_CP085885:183807-184088 [Pseudomonas sp. HN2]
MTYKLEFLPSAHKEWNKLGHTLREQFKKKLGERLKLPRIPADALHSMPDCYKIKLKASGYRLVYQVIDERVVVSVLAVGK
RERSSVYDAAKNR

Nucleotide

• Length: 282 bp

>T222225 NZ_CP117723:c4664664-4664293 [Enterobacter asburiae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTTTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAGTTGCCCGTTAATCTCACGCAA
TACGAAAGACTTTTTGGGCATCACCGGGGTCGTTTCACCTTACCAGCTGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8806.13 Da
• Isoelectric Point: 4.4988

>AT222225 WP_041477418.1 NZ_CP085885:183572-183817 [Pseudomonas sp. HN2]
MQSVLADMAVSVSELKKNPTAVLNGAHGGAVAVLNHNRVMGYMVPAEVYEAMMERLDDLELADIVRSRLHETPISVSLDD
L

Nucleotide

• Length: 246 bp

>AT222225 NZ_CP117723:c4664909-4664667 [Enterobacter asburiae]
ATGATTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGAAGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A2U1R4X8