Detailed information of TA system TA222224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 6166129..6166782
• Replicon: chromosome
• Accession: NZ_CP085876
• Organism: Brevibacillus sp. DP1.3A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: J2PLP3
• Locus tag: HP399_RS28510
• Protein ID: WP_005830205.1
• Coordinates: 6166129..6166479 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: J2GZH3
• Locus tag: HP399_RS28515
• Protein ID: WP_007721089.1
• Coordinates: 6166483..6166782 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP399_RS28485 (HP399_028530)	6162883..6163671	-	789	WP_173621356.1	RNA polymerase sigma factor SigB	-
HP399_RS28490 (HP399_028535)	6163640..6164134	-	495	WP_173621357.1	anti-sigma B factor RsbW	-
HP399_RS28495 (HP399_028540)	6164134..6164460	-	327	WP_173621358.1	anti-sigma factor antagonist	-
HP399_RS28500 (HP399_028545)	6164485..6165486	-	1002	WP_173621359.1	PP2C family protein-serine/threonine phosphatase	-
HP399_RS28505 (HP399_028550)	6165492..6165836	-	345	WP_228088576.1	anti-sigma regulatory factor	-
HP399_RS28510 (HP399_028555)	6166129..6166479	-	351	WP_005830205.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HP399_RS28515 (HP399_028560)	6166483..6166782	-	300	WP_007721089.1	CopG family ribbon-helix-helix protein	Antitoxin
HP399_RS28520 (HP399_028565)	6167012..6168214	-	1203	WP_173621361.1	alanine racemase	-
HP399_RS28525 (HP399_028570)	6168217..6168657	-	441	WP_007721092.1	hypothetical protein	-
HP399_RS28530 (HP399_028575)	6168842..6170248	-	1407	WP_173621362.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-52)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12917.86 Da
• Isoelectric Point: 4.8435

>T222224 WP_005830205.1 NZ_CP085876:c6166479-6166129 [Brevibacillus sp. DP1.3A]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T222224 NZ_CP117723:c3899708-3899298 [Enterobacter asburiae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTTTTGCTGATGCCGTGGCCGCTGAGCTATACGCCGCTGTGGCTGCTGTTACTGTCGTTTGTCGTTTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATTAAATTACTGATGGATTCCCGCCTTCGCTGGCAGGGTAAA
GAGTGGGATATTCTCGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11309.19 Da
• Isoelectric Point: 10.2101

>AT222224 WP_007721089.1 NZ_CP085876:c6166782-6166483 [Brevibacillus sp. DP1.3A]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLNMASE
AFGAEEEADHTLVRLVSGV

Nucleotide

• Length: 300 bp

>AT222224 NZ_CP117723:c3899955-3899689 [Enterobacter asburiae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: J2PLP3

Antitoxin

• AlphaFold DB: J2GZH3