Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 564447..565100 Replicon chromosome
Accession NZ_CP085874
Organism Brevibacillus sp. HD3.3A

Toxin (Protein)


Gene name mazF Uniprot ID J2PLP3
Locus tag HP435_RS03015 Protein ID WP_005830205.1
Coordinates 564750..565100 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A3M8AYL7
Locus tag HP435_RS03010 Protein ID WP_025845698.1
Coordinates 564447..564746 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HP435_RS02990 (HP435_03005) 559720..560757 + 1038 WP_122966581.1 outer membrane lipoprotein-sorting protein -
HP435_RS02995 (HP435_03010) 560956..562401 + 1446 WP_229049804.1 hypothetical protein -
HP435_RS03000 (HP435_03015) 562623..563063 + 441 WP_122966579.1 hypothetical protein -
HP435_RS03005 (HP435_03020) 563066..564268 + 1203 WP_173599859.1 alanine racemase -
HP435_RS03010 (HP435_03025) 564447..564746 + 300 WP_025845698.1 CopG family ribbon-helix-helix protein Antitoxin
HP435_RS03015 (HP435_03030) 564750..565100 + 351 WP_005830205.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HP435_RS03020 (HP435_03035) 565406..565762 + 357 WP_229049930.1 anti-sigma regulatory factor -
HP435_RS03025 (HP435_03040) 565756..566757 + 1002 WP_122966576.1 PP2C family protein-serine/threonine phosphatase -
HP435_RS03030 (HP435_03045) 566783..567109 + 327 WP_063229461.1 anti-sigma factor antagonist -
HP435_RS03035 (HP435_03050) 567109..567603 + 495 WP_122966575.1 anti-sigma B factor RsbW -
HP435_RS03040 (HP435_03055) 567572..568369 + 798 WP_122966574.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-52)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12917.86 Da        Isoelectric Point: 4.8435

>T222223 WP_005830205.1 NZ_CP085874:564750-565100 [Brevibacillus sp. HD3.3A]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVNESLQISLGLIDF

Download         Length: 351 bp

>T222223 NZ_CP117723:3674317-3674616 [Enterobacter asburiae]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGATATTCCTCAATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11323.26 Da        Isoelectric Point: 10.3594

>AT222223 WP_025845698.1 NZ_CP085874:564447-564746 [Brevibacillus sp. HD3.3A]
MVGGGFVLSKSNTKRIMISLPQHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRTIRETMQKGYMEMAKINLKMASE
AFGAEEEADHTLVRLVSGV

Download         Length: 300 bp

>AT222223 NZ_CP117723:3674627-3674974 [Enterobacter asburiae]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAATAATCGCAAGCTGA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2PLP3

Antitoxin

Source ID Structure
AlphaFold DB A0A3M8AYL7

References