Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 53231..54141 Replicon plasmid pMV-u1-SK2-O-a
Accession NZ_CP085867
Organism Klebsiella pneumoniae strain MV-u1-SK2-O

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag LKZ97_RS26290 Protein ID WP_004144067.1
Coordinates 53671..54141 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag LKZ97_RS26285 Protein ID WP_023287184.1
Coordinates 53231..53674 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKZ97_RS26270 (LKZ97_26320) 48529..49710 + 1182 WP_023287181.1 hypothetical protein -
LKZ97_RS26275 (LKZ97_26325) 49707..51935 + 2229 WP_023287182.1 ThiF family adenylyltransferase -
LKZ97_RS26280 (LKZ97_26330) 51988..53130 + 1143 Protein_54 translesion error-prone DNA polymerase V subunit UmuC -
LKZ97_RS26285 (LKZ97_26335) 53231..53674 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
LKZ97_RS26290 (LKZ97_26340) 53671..54141 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
LKZ97_RS26295 (LKZ97_26345) 54228..55196 + 969 WP_000654811.1 IS5 family transposase -
LKZ97_RS26300 (LKZ97_26350) 55316..55576 - 261 WP_017901409.1 hypothetical protein -
LKZ97_RS26305 (LKZ97_26355) 56150..56923 - 774 Protein_59 integrase core domain-containing protein -
LKZ97_RS26310 (LKZ97_26360) 56927..57109 - 183 Protein_60 IS481 family transposase -
LKZ97_RS26315 (LKZ97_26365) 57100..58068 + 969 WP_167876146.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sul2 / aph(3'')-Ib / aph(6)-Id / qnrS1 / catA2 - 1..202882 202882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T222222 WP_004144067.1 NZ_CP085867:53671-54141 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T222222 NZ_CP117723:c3182734-3182447 [Enterobacter asburiae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT222222 WP_023287184.1 NZ_CP085867:53231-53674 [Klebsiella pneumoniae]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT222222 NZ_CP117723:c3182973-3182734 [Enterobacter asburiae]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References