Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5242617..5243242 Replicon chromosome
Accession NZ_CP085866
Organism Klebsiella pneumoniae strain MV-u1-SK2-O

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LKZ97_RS25650 Protein ID WP_002882817.1
Coordinates 5242617..5243000 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LKZ97_RS25655 Protein ID WP_004150355.1
Coordinates 5243000..5243242 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKZ97_RS25635 (LKZ97_25685) 5239983..5240885 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LKZ97_RS25640 (LKZ97_25690) 5240882..5241517 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LKZ97_RS25645 (LKZ97_25695) 5241514..5242443 + 930 WP_046654801.1 formate dehydrogenase accessory protein FdhE -
LKZ97_RS25650 (LKZ97_25700) 5242617..5243000 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LKZ97_RS25655 (LKZ97_25705) 5243000..5243242 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LKZ97_RS25660 (LKZ97_25710) 5243447..5244364 + 918 WP_004181612.1 alpha/beta hydrolase -
LKZ97_RS25665 (LKZ97_25715) 5244378..5245319 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LKZ97_RS25670 (LKZ97_25720) 5245364..5245801 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LKZ97_RS25675 (LKZ97_25725) 5245798..5246658 - 861 WP_046654802.1 virulence factor BrkB family protein -
LKZ97_RS25680 (LKZ97_25730) 5246652..5247251 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T222221 WP_002882817.1 NZ_CP085866:c5243000-5242617 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T222221 NZ_CP117723:c3001390-3001037 [Enterobacter asburiae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGTACTGGCATC
ATGCTTTGTGCTGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCAGGAATTCAC
TCACTGGCTGGATAGATTAAAAGAGAGGGAATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT222221 WP_004150355.1 NZ_CP085866:c5243242-5243000 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT222221 NZ_CP117723:c3001032-3000730 [Enterobacter asburiae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGTGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCGGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGACGGCTCGCACTATGAGTTTGTGCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References