Detailed information of TA system TA222220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4769711..4770227
• Replicon: chromosome
• Accession: NZ_CP085866
• Organism: Klebsiella pneumoniae strain MV-u1-SK2-O

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: LKZ97_RS23395
• Protein ID: WP_040216106.1
• Coordinates: 4769711..4769995 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: LKZ97_RS23400
• Protein ID: WP_002886901.1
• Coordinates: 4769985..4770227 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKZ97_RS23370 (LKZ97_23420)	4765128..4765391	-	264	WP_014908078.1	PTS sugar transporter subunit IIB	-
LKZ97_RS23375 (LKZ97_23425)	4765521..4765694	+	174	WP_032425422.1	hypothetical protein	-
LKZ97_RS23380 (LKZ97_23430)	4765697..4766440	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
LKZ97_RS23385 (LKZ97_23435)	4766797..4768935	+	2139	WP_004186701.1	anaerobic ribonucleoside-triphosphate reductase	-
LKZ97_RS23390 (LKZ97_23440)	4769243..4769707	+	465	WP_032418146.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
LKZ97_RS23395 (LKZ97_23445)	4769711..4769995	-	285	WP_040216106.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LKZ97_RS23400 (LKZ97_23450)	4769985..4770227	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
LKZ97_RS23405 (LKZ97_23455)	4770305..4772215	-	1911	WP_040216105.1	PRD domain-containing protein	-
LKZ97_RS23410 (LKZ97_23460)	4772238..4773392	-	1155	WP_002886900.1	lactonase family protein	-
LKZ97_RS23415 (LKZ97_23465)	4773459..4774199	-	741	WP_004186692.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11097.94 Da
• Isoelectric Point: 10.4962

>T222220 WP_040216106.1 NZ_CP085866:c4769995-4769711 [Klebsiella pneumoniae]
MTYELAFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T222220 NZ_CP117723:c2998653-2998321 [Enterobacter asburiae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCGGTAGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACCGTTTCACTGGAAGGGGCGGGAACAACAACAACGGGCGTTATTCGCTGCGATCAGCCCAGAACCATT
GATATGTCTGCCCGGAACGGCATGCGGCTGGAACGTATACCTGACGCGGTTATAAATGAAGTGCTTGCCAGGCTGGATGC
GATTCTGAATTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT222220 WP_002886901.1 NZ_CP085866:c4770227-4769985 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT222220 NZ_CP117723:c2998911-2998654 [Enterobacter asburiae]
ATGTATACAACTCGTCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAACT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAGCGGCCTCACTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCTGAAATGAGCGAAGAGGAGCGGGAATGGATCGATGCGCCTGCGGTG
GGCAGGGAGATCCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GLP0