Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4066196..4066815 Replicon chromosome
Accession NZ_CP085866
Organism Klebsiella pneumoniae strain MV-u1-SK2-O

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LKZ97_RS20080 Protein ID WP_002892050.1
Coordinates 4066597..4066815 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LKZ97_RS20075 Protein ID WP_002892066.1
Coordinates 4066196..4066570 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKZ97_RS20065 (LKZ97_20110) 4061348..4062541 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LKZ97_RS20070 (LKZ97_20115) 4062564..4065710 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LKZ97_RS20075 (LKZ97_20120) 4066196..4066570 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LKZ97_RS20080 (LKZ97_20125) 4066597..4066815 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LKZ97_RS20085 (LKZ97_20130) 4066974..4067540 + 567 WP_002892030.1 maltose O-acetyltransferase -
LKZ97_RS20090 (LKZ97_20135) 4067512..4067652 - 141 WP_004147370.1 hypothetical protein -
LKZ97_RS20095 (LKZ97_20140) 4067673..4068143 + 471 WP_048337480.1 YlaC family protein -
LKZ97_RS20100 (LKZ97_20145) 4068118..4069569 - 1452 WP_048337479.1 PLP-dependent aminotransferase family protein -
LKZ97_RS20105 (LKZ97_20150) 4069670..4070368 + 699 WP_002892021.1 GNAT family protein -
LKZ97_RS20110 (LKZ97_20155) 4070365..4070505 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LKZ97_RS20115 (LKZ97_20160) 4070505..4070768 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T222218 WP_002892050.1 NZ_CP085866:4066597-4066815 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T222218 NZ_CP117723:2329894-2330181 [Enterobacter asburiae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT222218 WP_002892066.1 NZ_CP085866:4066196-4066570 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT222218 NZ_CP117723:2329655-2329894 [Enterobacter asburiae]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References