Detailed information of TA system TA222216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2588196..2589784
• Replicon: chromosome
• Accession: NZ_CP085866
• Organism: Klebsiella pneumoniae strain MV-u1-SK2-O

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LKZ97_RS12720
• Protein ID: WP_048337706.1
• Coordinates: 2588196..2589518 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LKZ97_RS12725
• Protein ID: WP_002904790.1
• Coordinates: 2589518..2589784 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKZ97_RS12705 (LKZ97_12720)	2583252..2585753	+	2502	WP_048337707.1	fimbria/pilus outer membrane usher protein	-
LKZ97_RS12710 (LKZ97_12725)	2585741..2586745	+	1005	WP_048265557.1	fimbrial protein	-
LKZ97_RS12715 (LKZ97_12730)	2586887..2588179	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LKZ97_RS12720 (LKZ97_12735)	2588196..2589518	-	1323	WP_048337706.1	type II toxin-antitoxin system HipA family toxin	Toxin
LKZ97_RS12725 (LKZ97_12740)	2589518..2589784	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LKZ97_RS12730 (LKZ97_12745)	2590050..2590376	-	327	WP_048265555.1	hydroxyisourate hydrolase	-
LKZ97_RS12735 (LKZ97_12750)	2590373..2590873	-	501	WP_048337705.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LKZ97_RS12740 (LKZ97_12755)	2590851..2592230	-	1380	WP_048337704.1	nucleobase:cation symporter-2 family protein	-
LKZ97_RS12745 (LKZ97_12760)	2592576..2593730	+	1155	WP_048337703.1	FAD-dependent urate hydroxylase HpxO	-
LKZ97_RS12750 (LKZ97_12765)	2593711..2594634	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49127.41 Da
• Isoelectric Point: 7.5357

>T222216 WP_048337706.1 NZ_CP085866:c2589518-2588196 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDVISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T222216 NZ_CP117723:c1054422-1054204 [Enterobacter asburiae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGGCGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT222216 WP_002904790.1 NZ_CP085866:c2589784-2589518 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT222216 NZ_CP117723:c1054824-1054450 [Enterobacter asburiae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACATCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCGCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8