Detailed information of TA system TA022221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1769408..1770021
• Replicon: chromosome
• Accession: NC_009565
• Organism: Mycobacterium tuberculosis F11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: TBFG_RS08285
• Protein ID: WP_003407786.1
• Coordinates: 1769617..1770021 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8UJ46
• Locus tag: TBFG_RS08280
• Protein ID: WP_009935474.1
• Coordinates: 1769408..1769611 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBFG_RS08250	1764813..1765193	+	381	WP_003407768.1	fumarate reductase subunit C	-
TBFG_RS08255	1765190..1765567	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
TBFG_RS08260	1765635..1766243	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
TBFG_RS08265	1766427..1767575	+	1149	Protein_1614	MMPL family transporter	-
TBFG_RS08270	1767585..1768031	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
TBFG_RS08275	1768066..1769355	+	1290	WP_003407781.1	threonine ammonia-lyase	-
TBFG_RS08280	1769408..1769611	+	204	WP_009935474.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBFG_RS08285	1769617..1770021	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
TBFG_RS08290	1770038..1771780	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
TBFG_RS08295	1771773..1774070	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T22221 WP_003407786.1 NC_009565:1769617-1770021 [Mycobacterium tuberculosis F11]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T22221 NC_009565:1769617-1770021 [Mycobacterium tuberculosis F11]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7501.48 Da
• Isoelectric Point: 4.3951

>AT22221 WP_009935474.1 NC_009565:1769408-1769611 [Mycobacterium tuberculosis F11]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT22221 NC_009565:1769408-1769611 [Mycobacterium tuberculosis F11]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V

Antitoxin

• AlphaFold DB: A0A0E8UJ46