Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 727738..728513 Replicon chromosome
Accession NZ_CP085866
Organism Klebsiella pneumoniae strain MV-u1-SK2-O

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1W1JAQ8
Locus tag LKZ97_RS03615 Protein ID WP_009308645.1
Coordinates 728028..728513 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag LKZ97_RS03610 Protein ID WP_004150912.1
Coordinates 727738..728031 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKZ97_RS03590 (LKZ97_03590) 722947..723549 - 603 WP_004174410.1 short chain dehydrogenase -
LKZ97_RS03595 (LKZ97_03595) 723647..724558 + 912 WP_004188550.1 LysR family transcriptional regulator -
LKZ97_RS03600 (LKZ97_03600) 724559..725707 - 1149 WP_080934664.1 PLP-dependent aspartate aminotransferase family protein -
LKZ97_RS03605 (LKZ97_03605) 725718..727094 - 1377 WP_004181304.1 cystathionine beta-synthase -
LKZ97_RS03610 (LKZ97_03610) 727738..728031 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
LKZ97_RS03615 (LKZ97_03615) 728028..728513 + 486 WP_009308645.1 GNAT family N-acetyltransferase Toxin
LKZ97_RS03620 (LKZ97_03620) 728692..729144 - 453 WP_080816334.1 hypothetical protein -
LKZ97_RS03625 (LKZ97_03625) 729259..729478 - 220 Protein_711 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17595.60 Da        Isoelectric Point: 8.5144

>T222206 WP_009308645.1 NZ_CP085866:728028-728513 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPDPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T222206 NZ_CP117721:c67718-67338 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT222206 WP_004150912.1 NZ_CP085866:727738-728031 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT222206 NZ_CP117721:c67939-67718 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W1JAQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References