Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5185106..5185731 Replicon chromosome
Accession NZ_CP085863
Organism Klebsiella pneumoniae strain MV-v4-SK2-O

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag LKZ99_RS25355 Protein ID WP_064279144.1
Coordinates 5185106..5185489 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LKZ99_RS25360 Protein ID WP_004150355.1
Coordinates 5185489..5185731 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKZ99_RS25340 (LKZ99_25405) 5182472..5183374 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LKZ99_RS25345 (LKZ99_25410) 5183371..5184006 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LKZ99_RS25350 (LKZ99_25415) 5184003..5184932 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LKZ99_RS25355 (LKZ99_25420) 5185106..5185489 - 384 WP_064279144.1 type II toxin-antitoxin system VapC family toxin Toxin
LKZ99_RS25360 (LKZ99_25425) 5185489..5185731 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LKZ99_RS25365 (LKZ99_25430) 5185936..5186853 + 918 WP_048996318.1 alpha/beta hydrolase -
LKZ99_RS25370 (LKZ99_25435) 5186867..5187808 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LKZ99_RS25375 (LKZ99_25440) 5187853..5188290 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LKZ99_RS25380 (LKZ99_25445) 5188287..5189147 - 861 WP_023280688.1 virulence factor BrkB family protein -
LKZ99_RS25385 (LKZ99_25450) 5189141..5189740 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-113)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14476.75 Da        Isoelectric Point: 8.5338

>T222204 WP_064279144.1 NZ_CP085863:c5185489-5185106 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPRVVTPYEIHPE

Download         Length: 384 bp

>T222204 NZ_CP117720:3991737-3991889 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT222204 WP_004150355.1 NZ_CP085863:c5185731-5185489 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT222204 NZ_CP117720:c3991688-3991631 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References