Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4013790..4014409 Replicon chromosome
Accession NZ_CP085863
Organism Klebsiella pneumoniae strain MV-v4-SK2-O

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LKZ99_RS19815 Protein ID WP_002892050.1
Coordinates 4014191..4014409 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LKZ99_RS19810 Protein ID WP_002892066.1
Coordinates 4013790..4014164 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKZ99_RS19800 (LKZ99_19855) 4008942..4010135 + 1194 WP_086519390.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LKZ99_RS19805 (LKZ99_19860) 4010158..4013304 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LKZ99_RS19810 (LKZ99_19865) 4013790..4014164 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LKZ99_RS19815 (LKZ99_19870) 4014191..4014409 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LKZ99_RS19820 (LKZ99_19875) 4014568..4015134 + 567 WP_002892030.1 maltose O-acetyltransferase -
LKZ99_RS19825 (LKZ99_19880) 4015106..4015246 - 141 WP_004147370.1 hypothetical protein -
LKZ99_RS19830 (LKZ99_19885) 4015267..4015737 + 471 WP_002892026.1 YlaC family protein -
LKZ99_RS19835 (LKZ99_19890) 4015712..4017163 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LKZ99_RS19840 (LKZ99_19895) 4017264..4017962 + 699 WP_002892021.1 GNAT family protein -
LKZ99_RS19845 (LKZ99_19900) 4017959..4018099 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LKZ99_RS19850 (LKZ99_19905) 4018099..4018362 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T222201 WP_002892050.1 NZ_CP085863:4014191-4014409 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T222201 NZ_CP117720:2714114-2714221 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGTGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT222201 WP_002892066.1 NZ_CP085863:4013790-4014164 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT222201 NZ_CP117720:c2714061-2714000 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References