Detailed information of TA system TA222200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2591404..2592992
• Replicon: chromosome
• Accession: NZ_CP085863
• Organism: Klebsiella pneumoniae strain MV-v4-SK2-O

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LKZ99_RS12730
• Protein ID: WP_064159736.1
• Coordinates: 2591404..2592726 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: LKZ99_RS12735
• Protein ID: WP_002904790.1
• Coordinates: 2592726..2592992 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKZ99_RS12715 (LKZ99_12740)	2586460..2588961	+	2502	WP_086519589.1	fimbria/pilus outer membrane usher protein	-
LKZ99_RS12720 (LKZ99_12745)	2588949..2589953	+	1005	WP_004184029.1	fimbrial protein	-
LKZ99_RS12725 (LKZ99_12750)	2590095..2591387	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LKZ99_RS12730 (LKZ99_12755)	2591404..2592726	-	1323	WP_064159736.1	type II toxin-antitoxin system HipA family toxin	Toxin
LKZ99_RS12735 (LKZ99_12760)	2592726..2592992	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LKZ99_RS12740 (LKZ99_12765)	2593620..2593946	-	327	WP_086519588.1	hydroxyisourate hydrolase	-
LKZ99_RS12745 (LKZ99_12770)	2593943..2594443	-	501	WP_023282573.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LKZ99_RS12750 (LKZ99_12775)	2594421..2595800	-	1380	WP_086519587.1	nucleobase:cation symporter-2 family protein	-
LKZ99_RS12755 (LKZ99_12780)	2596146..2597300	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49187.51 Da
• Isoelectric Point: 7.5357

>T222200 WP_064159736.1 NZ_CP085863:c2592726-2591404 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSLRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T222200 NZ_CP117720:2713578-2713685 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT222200 WP_002904790.1 NZ_CP085863:c2592992-2592726 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT222200 NZ_CP117720:c2713531-2713465 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: A0A0H3GXD8