Detailed information of TA system TA222185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3832860..3833516
• Replicon: chromosome
• Accession: NZ_CP085860
• Organism: Serratia marcescens strain MV-u1-SK1-O

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A656VD00
• Locus tag: LKZ96_RS18310
• Protein ID: WP_025303864.1
• Coordinates: 3832860..3833249 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8G2LCA8
• Locus tag: LKZ96_RS18315
• Protein ID: WP_004941563.1
• Coordinates: 3833253..3833516 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LKZ96_RS18295 (LKZ96_18320)	3829327..3830757	+	1431	WP_213928385.1	MFS transporter	-
LKZ96_RS18300 (LKZ96_18325)	3830754..3832136	+	1383	WP_049204856.1	two-component system sensor histidine kinase BaeS	-
LKZ96_RS18305 (LKZ96_18330)	3832136..3832852	+	717	WP_004941568.1	two-component system response regulator BaeR	-
LKZ96_RS18310 (LKZ96_18335)	3832860..3833249	-	390	WP_025303864.1	type II toxin-antitoxin system VapC family toxin	Toxin
LKZ96_RS18315 (LKZ96_18340)	3833253..3833516	-	264	WP_004941563.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LKZ96_RS18320 (LKZ96_18345)	3833854..3834192	+	339	WP_016926703.1	YegP family protein	-
LKZ96_RS18325 (LKZ96_18350)	3834371..3835723	+	1353	WP_025303865.1	tRNA 5-hydroxyuridine modification protein YegQ	-
LKZ96_RS18330 (LKZ96_18355)	3835940..3836161	-	222	WP_141998673.1	hypothetical protein	-
LKZ96_RS18335 (LKZ96_18360)	3836191..3837096	+	906	WP_197791343.1	lipid kinase YegS	-
LKZ96_RS18340 (LKZ96_18365)	3837265..3838479	+	1215	WP_025303867.1	D-galactonate dehydratase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-119)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14351.67 Da
• Isoelectric Point: 9.5570

>T222185 WP_025303864.1 NZ_CP085860:c3833249-3832860 [Serratia marcescens]
MYMFDTNTVSHLFRQHPQVLNVMQKLPPSAVCISSVTEAELLYGVAKRRNKALQSMVEAFLAAVTVYAWDREAARCYGEM
RANMGRKGKIMGAMDQLIAAHAQSRGATLVTNDRAFAMVPGLAVEDWTR

Nucleotide

• Length: 390 bp

>T222185 NZ_CP117717:c4734701-4734390 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10062.31 Da
• Isoelectric Point: 4.6702

>AT222185 WP_004941563.1 NZ_CP085860:c3833516-3833253 [Serratia marcescens]
MERIAKLFKNGRNQAVRLPVEFEFDTDRVYIRRDREGNVILSKYPAKPDSWAPLLSIIAQTPVPQEFLGAEDRQQGVADR
DPFSEEE

Nucleotide

• Length: 264 bp

>AT222185 NZ_CP117717:c4734389-4734099 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A656VD00