Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1076865..1077487 Replicon chromosome
Accession NZ_CP085860
Organism Serratia marcescens strain MV-u1-SK1-O

Toxin (Protein)


Gene name Hha Uniprot ID A0A8B4G7F9
Locus tag LKZ96_RS05050 Protein ID WP_004940313.1
Coordinates 1076865..1077068 (-) Length 68 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A2X2G5J9
Locus tag LKZ96_RS05055 Protein ID WP_004940312.1
Coordinates 1077119..1077487 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LKZ96_RS05020 (LKZ96_05030) 1072564..1072902 + 339 WP_004940327.1 P-II family nitrogen regulator -
LKZ96_RS05025 (LKZ96_05035) 1072939..1074225 + 1287 WP_038628958.1 ammonium transporter AmtB -
LKZ96_RS05030 (LKZ96_05040) 1074316..1075179 - 864 WP_016928847.1 acyl-CoA thioesterase II -
LKZ96_RS05035 (LKZ96_05045) 1075411..1075902 + 492 WP_025301777.1 YbaY family lipoprotein -
LKZ96_RS05040 (LKZ96_05050) 1075967..1076281 - 315 WP_025301778.1 MGMT family protein -
LKZ96_RS05050 (LKZ96_05060) 1076865..1077068 - 204 WP_004940313.1 HHA domain-containing protein Toxin
LKZ96_RS05055 (LKZ96_05065) 1077119..1077487 - 369 WP_004940312.1 Hha toxicity modulator TomB Antitoxin
LKZ96_RS05060 (LKZ96_05070) 1077646..1077999 - 354 WP_025301779.1 hypothetical protein -
LKZ96_RS05065 (LKZ96_05075) 1078408..1079121 + 714 WP_060447642.1 ABC transporter ATP-binding protein -
LKZ96_RS05070 (LKZ96_05080) 1079118..1079975 + 858 WP_043135664.1 metal ABC transporter permease -
LKZ96_RS05075 (LKZ96_05085) 1080001..1080879 + 879 WP_025301782.1 metal ABC transporter substrate-binding protein -
LKZ96_RS05080 (LKZ96_05090) 1080990..1081130 - 141 WP_004940304.1 type B 50S ribosomal protein L36 -
LKZ96_RS05085 (LKZ96_05095) 1081143..1081397 - 255 WP_004940303.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(8-64)

Antitoxin

Biofilm formation regulator YbaJ

(1-118)


Sequences

Toxin        


Download         Length: 68 a.a.        Molecular weight: 8107.37 Da        Isoelectric Point: 6.9756

>T222172 WP_004940313.1 NZ_CP085860:c1077068-1076865 [Serratia marcescens]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTSVWKYVR

Download         Length: 204 bp

>T222172 NZ_CP117717:1063926-1064261 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 14261.95 Da        Isoelectric Point: 4.4314

>AT222172 WP_004940312.1 NZ_CP085860:c1077487-1077119 [Serratia marcescens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEDICTTMKT

Download         Length: 369 bp

>AT222172 NZ_CP117717:1063678-1063926 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4G7F9

Antitoxin

Source ID Structure
AlphaFold DB A0A2X2G5J9

References