Detailed information of TA system TA222168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 15222..16026
• Replicon: plasmid pLH214-1
• Accession: NZ_CP085859
• Organism: Vibrio parahaemolyticus strain LH214

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: JK088_RS24485
• Protein ID: WP_268206397.1
• Coordinates: 15222..15752 (-)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: JK088_RS24490
• Protein ID: WP_047626400.1
• Coordinates: 15757..16026 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK088_RS24455 (JK088_24445)	10294..10575	+	282	WP_047626394.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JK088_RS24460 (JK088_24450)	10575..10877	+	303	WP_047626395.1	HigA family addiction module antitoxin	-
JK088_RS24465 (JK088_24455)	12543..13502	+	960	WP_268206420.1	plasmid segregation protein ParM	-
JK088_RS24470 (JK088_24460)	13545..13931	+	387	WP_049001901.1	plasmid partitioning/stability family protein	-
JK088_RS24475 (JK088_24465)	14138..14761	+	624	WP_032639997.1	ParA family partition ATPase	-
JK088_RS24480 (JK088_24470)	14758..14976	+	219	WP_232101232.1	plasmid partition protein ParG	-
JK088_RS24485 (JK088_24475)	15222..15752	-	531	WP_268206397.1	GNAT family N-acetyltransferase	Toxin
JK088_RS24490 (JK088_24480)	15757..16026	-	270	WP_047626400.1	DUF1778 domain-containing protein	Antitoxin
JK088_RS24495 (JK088_24485)	16643..17560	-	918	WP_050511221.1	RepB family plasmid replication initiator protein	-
JK088_RS24500 (JK088_24490)	18183..18656	+	474	WP_268206398.1	hypothetical protein	-
JK088_RS24505 (JK088_24495)	18681..18959	+	279	WP_268206399.1	hypothetical protein	-
JK088_RS24510 (JK088_24500)	19155..20075	-	921	WP_268206400.1	hypothetical protein	-
JK088_RS24515 (JK088_24505)	20120..20737	-	618	WP_268206401.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..89676	89676

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20220.02 Da
• Isoelectric Point: 6.4928

>T222168 WP_268206397.1 NZ_CP085859:c15752-15222 [Vibrio parahaemolyticus]
MRGLKVEIFSEEVEYDVSNFDCGEEYLNTFLTDHLKRQHIGKFLHGYVLVTREEKPRVLGYYTLSGSCFEKEYFPSKTQQ
KRVPYKNVPSVTLARLAIDSSIQRQGYGETLVTHAMKVVYHASQAVGIHGMFVEALSDDAKKFYLGLGFILLKRENSNAL
FYPTKSIEELFEAQDE

Nucleotide

• Length: 531 bp

>T222168 NZ_CP117716:c97150-96734 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10035.48 Da
• Isoelectric Point: 10.0883

>AT222168 WP_047626400.1 NZ_CP085859:c16026-15757 [Vibrio parahaemolyticus]
MSALKKQRIDLRLNEADKSMIEEAAAMTNQSISQFMVSTASERAAVIINQHRRLTLNEESWNAVMEAISNPPAPNDRLKK
AAKRLQNLE

Nucleotide

• Length: 270 bp

>AT222168 NZ_CP117716:c97377-97147 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA