Detailed information of TA system TA222160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4441080..4441894
• Replicon: chromosome
• Accession: NZ_CP085842
• Organism: Escherichia coli strain B95. delta A

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: LJ838_RS21595
• Protein ID: WP_001054376.1
• Coordinates: 4441637..4441894 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: LJ838_RS21590
• Protein ID: WP_001309181.1
• Coordinates: 4441080..4441625 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LJ838_RS21560 (4436771)	4436771..4438084	-	1314	WP_000460843.1	PTS sugar transporter subunit IIC SgcC	-
LJ838_RS21565 (4438096)	4438096..4438374	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
LJ838_RS21570 (4438371)	4438371..4439492	-	1122	WP_000010829.1	M42 family metallopeptidase	-
LJ838_RS21575 (4439737)	4439737..4439853	-	117	Protein_4200	VOC family protein	-
LJ838_RS21580 (4439891)	4439891..4440109	-	219	Protein_4201	hypothetical protein	-
LJ838_RS21585 (4440278)	4440278..4441024	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
LJ838_RS21590 (4441080)	4441080..4441625	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
LJ838_RS21595 (4441637)	4441637..4441894	-	258	WP_001054376.1	YjhX family toxin	Toxin
LJ838_RS21600 (4442385)	4442385..4442516	-	132	WP_001309182.1	hypothetical protein	-
LJ838_RS21605 (4442632)	4442632..4443872	+	1241	Protein_4206	helicase YjhR	-
LJ838_RS21610 (4444140)	4444140..4444345	-	206	Protein_4207	HNH endonuclease	-
LJ838_RS21615 (4444455)	4444455..4445435	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
LJ838_RS21620 (4445500)	4445500..4446606	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T222160 WP_001054376.1 NZ_CP085842:c4441894-4441637 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T222160 NZ_CP117714:3803507-3803785 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT222160 WP_001309181.1 NZ_CP085842:c4441625-4441080 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT222160 NZ_CP117714:3803244-3803504 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8