Detailed information of TA system TA022215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1243183..1243751
• Replicon: chromosome
• Accession: NC_009565
• Organism: Mycobacterium tuberculosis F11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: TBFG_RS05915
• Protein ID: WP_003405865.1
• Coordinates: 1243377..1243751 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: TBFG_RS05910
• Protein ID: WP_003405863.1
• Coordinates: 1243183..1243380 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBFG_RS05890	1239224..1239862	-	639	WP_003898730.1	lipid droplet-associated protein	-
TBFG_RS05895	1239952..1240959	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
TBFG_RS05900	1240976..1241959	-	984	WP_003898731.1	hypothetical protein	-
TBFG_RS05905	1242022..1243095	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
TBFG_RS05910	1243183..1243380	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
TBFG_RS05915	1243377..1243751	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
TBFG_RS05920	1243954..1244652	+	699	WP_003898733.1	hypothetical protein	-
TBFG_RS05925	1244770..1244955	+	186	WP_003901093.1	hypothetical protein	-
TBFG_RS05930	1244882..1245157	-	276	WP_003405867.1	hypothetical protein	-
TBFG_RS05935	1245400..1245723	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
TBFG_RS05940	1245738..1246448	-	711	WP_003911399.1	hypothetical protein	-
TBFG_RS22385	1246470..1246679	+	210	WP_003911400.1	hypothetical protein	-
TBFG_RS05950	1246631..1247338	-	708	Protein_1164	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T22215 WP_003405865.1 NC_009565:1243377-1243751 [Mycobacterium tuberculosis F11]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T22215 NC_009565:1243377-1243751 [Mycobacterium tuberculosis F11]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT22215 WP_003405863.1 NC_009565:1243183-1243380 [Mycobacterium tuberculosis F11]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT22215 NC_009565:1243183-1243380 [Mycobacterium tuberculosis F11]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07