Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 413481..414318 Replicon chromosome
Accession NZ_CP085842
Organism Escherichia coli strain B95. delta A

Toxin (Protein)


Gene name itaT Uniprot ID A0A140NB16
Locus tag LJ838_RS01965 Protein ID WP_000227786.1
Coordinates 413481..414023 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag LJ838_RS01970 Protein ID WP_001297137.1
Coordinates 414007..414318 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJ838_RS01945 (409008) 409008..409922 - 915 WP_227909691.1 2-dehydropantoate 2-reductase -
LJ838_RS01950 (410090) 410090..410581 + 492 WP_001138904.1 nucleotide binding protein YajQ -
LJ838_RS01955 (410709) 410709..412073 - 1365 WP_001000978.1 MFS transporter -
LJ838_RS01960 (412490) 412490..413425 + 936 WP_001297127.1 tetratricopeptide repeat protein -
LJ838_RS01965 (413481) 413481..414023 - 543 WP_000227786.1 GNAT family N-acetyltransferase Toxin
LJ838_RS01970 (414007) 414007..414318 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
LJ838_RS01975 (414503) 414503..415393 - 891 WP_000971336.1 heme o synthase -
LJ838_RS01980 (415405) 415405..415734 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
LJ838_RS01985 (415734) 415734..416348 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
LJ838_RS01990 (416338) 416338..418329 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
LJ838_RS01995 (418351) 418351..419298 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19794.98 Da        Isoelectric Point: 8.3395

>T222137 WP_000227786.1 NZ_CP085842:c414023-413481 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADSAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T222137 NZ_CP117698:c4490110-4489799 [Salmonella enterica subsp. enterica serovar London]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT222137 WP_001297137.1 NZ_CP085842:c414318-414007 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT222137 NZ_CP117698:c4489798-4489508 [Salmonella enterica subsp. enterica serovar London]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAGATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A140NB16

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References